کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9879479 | 1534760 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Different effect of beta-carotene on proliferation of prostate cancer cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
It was shown that high doses of beta-carotene (>30 μM) decrease proliferation of prostate cancer cells in vitro. However, it is rather doubtful whether such concentration of beta-carotene is really accessible at cellular level. We studied the effect of 3 and 10 μM beta-carotene on proliferation and gene expression in LNCaP and PC-3 prostate cancer cell lines. Beta - carotene-more efficiently absorbed from medium by androgen-sensitive LNCaP cells - increased proliferation of LNCaP cells whereas it had weaker effect on PC-3 cells. Initial global analysis of expression of genes in both cell lines treated with 10 μM beta-carotene (Affymetrix HG-U133A) showed remarkable differences in number of responsive genes. Their recognition allows for conclusion that differences between prostate cancer cell lines in response to beta-carotene treatment are due to various androgen sensitivities of LNCaP and PC-3 cells. Detailed analysis of expression of selected genes in beta-carotene treated LNCaP cells at the level of mRNA and protein indicated that the observed increase of proliferation could have been the result of slight induction of a few genes affecting proliferation (c-myc, c-jun) and apoptosis (bcl-2) with no significant effect on major cell cycle control genes (cdk2, RB, E2F-1).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1740, Issue 2, 30 May 2005, Pages 189-201
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1740, Issue 2, 30 May 2005, Pages 189-201
نویسندگان
Joanna DuliÅska, Dorota Gil, Jacek Zagajewski, Jadwiga Hartwich, Marek Bodzioch, Aldona DembiÅska-KieÄ, Thomas Langmann, Gerd Schmitz, Piotr Laidler,