کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9879500 | 1534761 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The type of collagen cross-link determines the reversibility of experimental skin fibrosis
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کلمات کلیدی
PBSMMP-1SSCTGF-β2MMPHPSHYPsystemic sclerosis - اسکلروز سیستمیکtransforming growth factor β2 - تبدیل فاکتور رشد β2Collagen degradation - تخریب کلاژنTIMP - زمانHyl - فریاد زدنPhosphate buffered saline - فسفات بافر شورFibrosis - فیبروز یا فساد الیافlysylpyridinoline - لیزیل پرییدینولینLysine - لیزینLYS - لیستmatrix metalloproteinase - ماتریکس متالوپروتئینازTissue inhibitor of metalloproteinases - مهار کننده های متالوپروتئینازهای بافتhydroxyproline - هیدروکسی پرولینhydroxylysylpyridinoline - هیدروکسیلیسیل پرییدینولینHydroxylysine - هیدروکسیلیسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fibrotic processes in humans are characterised by an excessive accumulation of collagen containing increased levels of hydroxyallysine-derived cross-links. The occurrence of these cross-links appears to be an important criterion in assessing the irreversibility of fibrosis. We hypothesise that increased hydroxyallysine cross-linking results in a collagenous matrix that is less susceptible to proteolytic degradation and therefore the collagen deposition is no longer reversible. In this report, we show that collagen matrices with increased hydroxyallysine cross-link levels were less susceptible to matrix metalloproteinase 1 degradation than are collagen matrices containing low hydroxyallysine levels. These data indicate that the type of collagen cross-link influences collagen catabolism. In vivo evidence for the importance of the cross-linking type in determining the reversibility of the fibrotic process was found using the bleomycin-induced skin fibrosis mouse model. The analysis of the accumulated collagen in the fibrotic skin of bleomycin-treated mice did not reveal an increase in hydroxyallysine cross-link levels. In concurrence with our hypothesis, the collagen accumulation resolved in time when the mice were no longer receiving bleomycin treatment, showing the reversibility of the fibrosis. In conclusion, our data indicate that the type of collagen cross-linking is an important factor in determining whether the outcome of the fibrotic process is reversible or not.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1740, Issue 1, 15 April 2005, Pages 60-67
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1740, Issue 1, 15 April 2005, Pages 60-67
نویسندگان
Annemarie J. van der Slot-Verhoeven, Ernst A. van Dura, Joline Attema, Bep Blauw, Jeroen DeGroot, Tom W.J. Huizinga, Anne-Marie Zuurmond, Ruud A. Bank,