کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886583 | 1537837 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increase in vulnerability to oxidative damage in cholesterol-modified erythrocytes exposed to t-BuOOH
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کلمات کلیدی
MDAPVPDcfTMA-DPHDPHtert-butylhydroperoxideMbCDBCDTBARS1,6-diphenyl-1,3,5-hexatriene - 1،6-دیفنیل-1،3،5-هگزیدریل2′,7′-dichlorofluorescein - 2 '، 7'-dichlorofluoresceinROS - ROSt-BuOOH - T-BuOOHOxidative damage - آسیب اکسیداتیوsphingomyelin - اسفنگومیلینPolyunsaturated fatty acids - اسید چرب اشباع نشدهPUFA - اسید چرب چند غیراشباعLipid oxidation - اکسیداسیون لیپیدیphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanolamine - فسفاتیدیلتانولامینPhospholipids - فسفولیپیدmalonyldialdehyde - مالونی دی آلدئیدmetHb - مت هابmethemoglobin - متهموگلوبینmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینthiobarbituric acid-reactive substances - مواد واکنش پذیر اسید تیوباربیتوریکHemoglobin - هموگلوبینtert-butyl hydroperoxide - هیدروپراکسید تری بوتیلpolyvinylpyrrolidone - پلی وینیل پیرولیدونcholesterol - کلسترولErythrocyte - گلبول قرمز یا اریتروسیتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
During the course of radical oxidation, cholesterol may exert seemingly contradictory effects. In order to gain a better understanding of the relationship between cholesterol levels and membrane susceptibility to oxidative damage induced by reactive oxygen species (ROS), here we analyze the integrity and structural stability of cholesterol-modified (enriched or depleted) and unmodified (control) erythrocytes exposed to tert-butyl hydroperoxide. The oxidant significantly increased ROS production, with almost complete oxidation of hemoglobin and a reduction in GSH content in the different erythrocyte groups at 2 mM concentration. These changes were accompanied by losses of cholesterol and total phospholipids, the main decreases being in phosphatidylethanolamine and phosphatidylcholine. The highest lipid loss was found in the cholesterol-depleted group. Fatty acid analyses revealed changes only in peroxidized cholesterol-modified erythrocytes, with decreases in linoleic and arachidonic acids. Fluorescence anisotropy studies showed an increase in the fluidity of the negatively charged surface of peroxidized control erythrocytes. Increased hemolysis and a positive correlation between cellular osmotic fragility and malondialdehyde contents were found in all peroxidized groups. These findings provide evidence that the modification of cholesterol levels in the erythrocyte membrane has provoking effects on peroxidation, with corresponding increases in oxidative damage in the treated cell, possibly as a consequence of lipid bilayer destabilization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1734, Issue 1, 1 May 2005, Pages 74-85
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1734, Issue 1, 1 May 2005, Pages 74-85
نویسندگان
Abel López-Revuelta, José I. Sánchez-Gallego, Angel Hernández-Hernández, Jesús Sánchez-Yagüe, Marcial Llanillo,