کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886591 | 1537838 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nuclear phospholipase C-β1b activation during G2/M and late G1 phase in nocodazole-synchronized HL-60 cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PLCPI3KPKCMAP kinase kinaseIns(1,4,5)P3 - Ins (1،4،5) P3MAPK - MAPKPtdIns(4,5)P2 - PtdIns (4،5) P2diacylglycerol - دیسیل گلیسیرینDAG - روزHL-60 cell - سلول HL-60Phosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازphosphatidylinositol 4,5-bisphosphate - فسفاتیدیلینوزیتول 4،5-بیسفسفاتphospholipase C - فسفولیپاز CMEK - مجاهدین خلقnocodazole - نوکودازولNuclei - هستهProtein kinase C - پروتئین کیناز سیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenCell cycle - چرخه سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, the activity of nuclear phosphatidylinositol-specific phosholipase C (PI-PLC) was investigated in HL-60 cells blocked at G2/M phase by the addition of nocodazole, and released into medium as synchronously progressing cells. Two peaks of an increase in the nuclear PI-PLC activities were detected; an early peak reached a maximum at 1 h after release from the nocodazole block, and a second increase was detected at 8.5 h after the release. Immunoprecipitation studies indicated that the increase in the activity was due to the activation of the nuclear PI-PLC-β1. Western blot analysis demonstrated no changes in the level of both a and b splicing variants of PI-PLC-β1 in the nuclei of cells isolated at either 1 h or 8.5 h after the block. However, an increase in the serine-phosphorylation of PI-PLC-β1b was detected in the nuclei of HL-60 cells isolated at 1 and 8.5 h after the block, and the presence of MEK-inhibitor PD98059 completely inhibited both the serine phosphorylation and the increase in the PI-PLC activities in vitro. The presence of PI-PLC inhibitor prevented the progression of HL-60 cells through the G1 into S phase of the cell cycle. These results demonstrate that two peaks of nuclear PI-PLC activities, which are due to a PD98059-sensitive phosphorylation of nuclear PLC-β1b on serine, occur at the G2/M and late G1 phase and are necessary for the progression of the cells through the cell cycle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1733, Issues 2â3, 15 April 2005, Pages 148-156
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1733, Issues 2â3, 15 April 2005, Pages 148-156
نویسندگان
Vesna LukinoviÆ-Å kudar, Lana ÄonlagiÆ, Hrvoje BanfÃÆ, Dora ViÅ¡njiÆ,