کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9908659 | 1547708 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5
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کلمات کلیدی
EGFREGFATDC5 cellsFGFFCSBSA - BSAMAPK - MAPKbovine serum albumin - آلبومین سرم گاوIntegrin - اینتگرینfetal calf serum - سرم گوساله جنینepidermal growth factor - عامل رشد اپیدرمیfibroblast growth factor - فاکتور رشد فیبروبلاستlaminin-5 - لامینین -5BMP - مدیریت فرایند کسب و کارBone morphogenetic protein - پروتئین مورفوژنیک استخوانmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenChondrogenesis - کندروژنیکEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Laminin-5 is an important basement membrane protein that regulates cell adhesion and motility. It was previously found that the γ2 chain of laminin-5 is transiently expressed in embryonic cartilage. This suggests a possible role of laminin-5 in chondrogenesis. Here, we examined this possibility using the murine teratocarcinoma cell line ATDC5. ATDC5 cells transiently and weakly expressed laminin-5 when they were stimulated for differentiation. Exogenous laminin-5 in either insoluble or soluble form strongly inhibited the differentiation phenotypes, i.e. formation of cartilaginous cell aggregates and production of chondrogenic marker proteins through its integrin-binding domain LG3 in the α3 chain. Laminin-5 had no effect on cell growth. In addition, we found that the laminin-5 with the 105-kDa, processed γ2 chain suppressed differentiation more strongly than one with the 150-kDa γ2 chain. This indicated that the proteolytic processing of γ2 chain regulated the activity of laminin-5. However, a γ2 chain short arm fragment had no effect on the chondrogenesis, and it rather suppressed the differentiation at excessive concentrations. These results suggest that laminin-5 and its processing modulate chondrogenic differentiation during development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 256-269
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 256-269
نویسندگان
Junko Hashimoto, Takashi Ogawa, Yoshiaki Tsubota, Kaoru Miyazaki,