کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9909092 | 1548433 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toxicogenomic analysis of gene expression changes in rat liver after a 28-day oral benzene exposure
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کلمات کلیدی
HNF6ALAS2EPHX1G6PCn.s.NQO1CyPGSTG6PDGSHUGTMPO - DFOHnf - HNFNAD(P)H dehydrogenase - NAD (P) هیدروژنازUDP-glucuronosyltransferases - UDP-گلوکورونوزیل ترانسفرازAryl hydrocarbon - آرویل هیدروکربنcytochrome p450 enzyme - آنزیم p450 سیتوکرومnot statistically significant - از لحاظ آماری معنی دار نیستbenzene - بنزنGene expression - بیان ژنNMR - تشدید مغناطیسی هستهای Hepatotoxicity - سمیت کبدToxicogenomics - سمژنگانشناسیNuclear magnetic resonance spectroscopy - طیف سنجی رزونانس مغناطیسی هسته ایhepatic nuclear factor - عامل هسته ای کبدMetabolomics - متابولومیکTranscriptomics - متن ترانهprincipal component - مولفه های اصلیmyeloperoxidase - میلوپراکسیداز cDNA microarrays - میکروآرایه های cDNAMicrosomal epoxide hydrolase - هیدرولاز اپوکسی میکروسیمالControl - کنترلGlutathione - گلوتاتیونglutathione-S-transferase - گلوتاتیون S-ترانسفرازglucose-6-phosphate dehydrogenase - گلوکز 6-فسفات دهیدروژنازglucose-6-phosphatase - گلوکز 6-فسفاتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Benzene is an industrial chemical, component of automobile exhaust and cigarette smoke. After hepatic bioactivation benzene induces bone marrow, blood and hepatic toxicity. Using a toxicogenomics approach this study analysed the effects of benzene at three dose levels on gene expression in the liver after 28 daily doses. NMR based metabolomics was used to assess benzene exposure by identification of characteristic benzene metabolite profiles in urine. The 28-day oral exposure to 200 and 800Â mg/kg/day but not 10Â mg/kg/day benzene-induced hematotoxicity in male Fisher rats. Additionally these upper dose levels slightly reduced body weight and increased relative liver weights. Changes in hepatic gene expression were identified with oligonucleotide microarrays at all dose levels including the 10Â mg/kg/day dose level where no toxicity was detected by other methods. The benzene-induced gene expression changes were related to pathways of biotransformation, glutathione synthesis, fatty acid and cholesterol metabolism and others. Some of the effects on gene expression observed here have previously been observed after induction of acute hepatic necrosis with bromobenzene and acetaminophen. In conclusion, changes in hepatic gene expression were found after treatment with benzene both at the toxic and non-toxic doses. The results from this study show that toxicogenomics identified hepatic effects of benzene exposure possibly related to toxicity. The findings aid to interpret the relevance of hepatic gene expression changes in response to exposure to xenobiotics. In addition, the results have the potential to inform on the mechanisms of response to benzene exposure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 575, Issues 1â2, 4 August 2005, Pages 85-101
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 575, Issues 1â2, 4 August 2005, Pages 85-101
نویسندگان
Wilbert H.M. Heijne, Diana Jonker, Rob H. Stierum, Ben van Ommen, John P. Groten,