کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9919027 | 1557567 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
علوم دارویی
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چکیده انگلیسی
Nanoparticles of calcium phosphate encapsulating plasmid DNA (pDNA) of size 100-120Â nm in diameter were prepared. XRD studies of these nanoparticles showed them to be crystalline in nature having hydroxyapatite structure. The maximum loading of pDNA and its release from nanoparticles were studied using gel electrophoresis. The time dependent size measurement of these particles demonstrated that these particles show strong aggregational behaviour in aqueous dispersion. Calcium phosphate nanoparticles were found to be dissolved even in low acidic buffer (pH 5.0) releasing the pDNA, which suggested that DNA release from these particles in the endosomal compartment was possible. In vitro transfection efficiency of these calcium phosphate nanoparticles was found to be higher than that of the commercial transfecting reagent Polyfect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 288, Issue 1, 6 January 2005, Pages 157-168
Journal: International Journal of Pharmaceutics - Volume 288, Issue 1, 6 January 2005, Pages 157-168
نویسندگان
Savita Bisht, Gajadhar Bhakta, Susmita Mitra, Amarnath Maitra,