Analysis of 14 highly informative SNP markers on X chromosome by TaqMan® SNP genotyping assay

ObjectiveTo genotype X-linked single nucleotide polymorphisms by TaqMan® SNP genotyping assay.Methods14 SNP markers on X chromosome were selected and genotyped by TaqMan® SNP genotyping assay in Chinese Han population samples.ResultsAccording to the results of population studies, deviations from Hardy–Weinberg equilibrium could be found at rs1299087 SNP loci, no deviations from Hardy–Weinberg equilibrium could be found at the other 13 SNP loci. The 14 X-SNPs were high informative. The overall power of discrimination (CPD) in females and in males were 0.999998379 and 0.999899, respectively. The combined exclusion probability in paternity testing for trios and duos were 0.9983 and 0.9788, respectively.ConclusionSNP typing by TaqMan® SNP genotyping assay is suitable for low-throughput application. The forensic efficiency parameters showed that the 14 X-linked SNPs are highly informative and seem to be useful for forensic genetics except rs1299087.