دانلود مقالات ISI درباره Iptg + ترجمه فارسی

Design and characterization of a recombinant colorimetric SAG1-alkaline phosphatase conjugate to detect specific antibody responses against Toxoplasma gondii

Keywords: T. gondii; Toxoplasma gondii; SAG1; surface antigen-1; AP; alkaline phosphatase; IPTG; isopropyl-β-d-thiogalactoside; PBS; phosphate-buffered saline; NBT; nitro-blue tetrazolium; BCIP; 5-bromo-4-choro-1-indolylphosphate; SDS-PAGE; sodium dodecyl sulfate

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

The amino acid residue L113 is involved in arginine kinase activity and structural stability

Keywords: AK; arginine kinase; CK; creatine kinase; PK; phosphogen kinase; IPTG; isopropyl-d-thiogalactopyranoside; ANS; 1-anilinonaphtalene-8-sulfonate; SEC; size exclusion chromatography; GdnHCl; guanidine hydrochloride; Emax; emission maximum wavelength of the i

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Post-translational membrane insertion of an endogenous YidC substrate

Keywords: DAG; 1-2-dioleoyl-sn-glycerol; DDM; n-dodecyl-β-maltoside; F0c; subunit c of the F0F1 ATP synthase; IPTG; isopropyl β-d-1-thiogalactopyranoside; LB; lysogeny broth; PK; proteinase K; RNC; ribosome nascent chain; SRP; signal recognition particle; TBS; tr

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Overexpression and characterization of a glucose-tolerant β-glucosidase from Thermotoga thermarum DSM 5069T with high catalytic efficiency of ginsenoside Rb1 to Rd

Keywords: IPTG; isopropyl-β-d-thiogalactopyranoside; GH; glycoside hydrolase; pNPGlu; p-nitrophenyl-β-d-glucopyranoside; pNPGal; p-nitrophenyl-β-d-galactopyranoside; pNPXyl; p-nitrophenyl-β-d-xylopyranoside; pNPAra; p-nitrophenyl-α-l-arabinofuranoside; Overexp

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion

Keywords: CA-asp; N-carbamoyl-l-aspartic acid; DHO; l-dihydroorotase; DNSSA; daptomycin-non-susceptible S. aureus; HRV 3C protease; recombinant type 14 3C protease from human rhinovirus; IPTG; isopropyl β-d-thiogalactopyranoside; LNSSA; linezolid-non-susceptible S

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Trp266 determines the binding specificity of a porcine aflatoxin B1 aldehyde reductase for aflatoxin B1-dialdehyde

Keywords: AFB1; aflatoxin B1; AFG1; aflatoxin G1; AFAR; aflatoxin B1 aldehyde reductase; AKR; aldo-keto reductase; AFBO; AFB1 exo-8,9-epoxide; 2-CBA; 2-carboxybenzaldehyde; SSA; succinic semialdehyde; WT; wide-type; IPTG; isopropyl-β-d-thiogalactoside; HPLC; high

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Molecular cloning, expression and antioxidant characterisation of a typical thioredoxin gene (AccTrx2) in Apis cerana cerana

Keywords: ROS; reactive oxygen species; Trxs; thioredoxins; UTR; untranslated region; ORF; open reading frame; RACE-PCR; rapid amplification of cDNA ends PCR; qRT-PCR; quantitative real-time PCR; IPTG; isopropyl-1-thio-β-galactopyranoside; EDTA; ethylenediaminetet

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Residues of a proposed gate region in type I ATP-binding cassette import systems are crucial for function as revealed by mutational analysis

Keywords: ABC; ATP-binding cassette; DDM; n-dodecyl-β-D-maltopyranoside; His-tag; hexahistidine tag; IPTG; isopropyl-β-D-thio-galactopyranoside; MOPS; 3-(N-morpholino)-propanesulfonic acid; OG; octyl-ß-D-glucopyranoside; PMSF; phenylmethylsulfonylfluoride; ABC t

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Detection of soluble co-factor dependent protein expression in vivo: Application to the 4′-phosphopantetheinyl transferase PptT from Mycobacterium tuberculosis

Keywords: ACP; acyl carrier protein; AcpS; acyl carrier protein synthase; AnTet; anhydrotetracycline; BSA; bovine serum albumin; CoA; Coenzyme A; DHFR; dihydrofolate reductase; DSF; differential scanning fluorimetry; DTT; dithiothreitol; GFP; green fluorescent prot

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Inherent properties of adenylosuccinate lyase could explain S-Ado/SAICAr ratio due to homozygous R426H and R303C mutations

Keywords: ADSL; adenylosuccinate lyase; WT; wild type; SAICAR; succinylaminoimidazolecarboxamide ribonucleotide; AICAR; aminoimidazolecarboxamide ribotide; SAMP; succinyladenosine monophosphate; AMP; adenosine monophosphate; PMR; psychomotor retardation; SAICAr; su

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Integral role of the I′-helix in the function of the “inactive” C-terminal domain of catalase-peroxidase (KatG)

Keywords: KatG; catalase-peroxidase; wtKatG; wild-type KatG; KatGN; separately expressed and isolated KatG N-terminal domain; KatGC; separately expressed and isolated KatG C-terminal domain; LL1; Large Loop 1; LL2; Large Loop 2; APx; ascorbate peroxidase; CcP; cy

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

High level prokaryotic expression of anti-Müllerian inhibiting substance type II receptor diabody, a new recombinant antibody for in vivo ovarian cancer imaging

Keywords: 12G4Db; dimeric diabody 12G4; 12G4Fm; monomeric format 12G4; Arg; arginine; BSA; bovine serum albumin; DTT; dithiothreitol; Gu-HCl; guanidine-HCl; IMAC; immobilized metal ion affinity chromatography; IPTG; isopropyl-β-d-thiogalactopyranoside; mAb; monocl

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Oligomerization of rice granule-bound starch synthase 1 modulates its activity regulation

Keywords: OsGBSS1; Oryza sativa granule-bound starch synthase 1; ADPG; adenosine diphosphate glucose; AGPase; ADP-glucose pyrophosphorylase; CAPS; 3-(cyclohexylamino)-1-propanesulfonic acid; DBE; starch debranching enzyme; DPA; days past anthesis; DTNB; 5,5′-dith

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions

Keywords: OAS; O-acetylserine; AVG; aminoethoxyvinylglycine; CBL; cystathionine β-lyase; CGL; cystathionine γ-lyase; CGS; cystathionine γ-synthase; Cth; cystathionine; Hcys; homocysteine; HO-HxoDH; d-2-Hydroxyisocaproate dehydrogenase; IPTG; Isopropyl-β-d-thiog

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Distinct roles of the R3H and RRM domains in poly(A)-specific ribonuclease structural integrity and catalysis

Keywords: ANS; 1-anilinonaphtalene-8-sulfonate; CD; circular dichroism; CTD; C-terminal domain; DTT; dithiothreitol; IPTG; isopropyl-1-thio-β-d-galactopyranoside; PARN; poly(A)-specific ribonuclease; p74; the full length PARN; p62; the N-terminal fragment of PARN

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Identification of novel glutelin subunits and a comparison of glutelin composition between japonica and indica rice (Oryza sativa L.)

Keywords: Japonica and indica rice; GluBX; Glutelin profile; Nutritional subunit composition; 2D-PAGE; two-dimension polyacrylamide gel electrophoresis; ACN; acetonitrile; CBB; Coomassie Brilliant Blue; DAF; days after flowering; DTT; dithiothreitol; HPLC; high per

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Identification of 15d-PGJ2 as an antagonist of farnesoid X receptor: Molecular modeling with biological evaluation

Keywords: 15d-PGJ2; 15-deoxy-Δ12,14-PGJ2; AA; arachidonic acid; PPARγ; peroxisome proliferator-activated receptor γ; FXR; farnesoid X receptor; SPR; surface plasmon resonance; FQ; fluorescence quenching; HTRF; homo time-resolved fluorescence; BSEP; bile-salt exp

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Phosphatidic acid and phosphoinositides facilitate liposome association of Yas3p and potentiate derepression of ARE1 (alkane-responsive element one)-mediated transcription control

Keywords: AID; auxin-inducible degron; ARE1; alkane-responsive element 1; bHLH; basic helix-loop-helix; DAG; diacylglycerol; DAGPP; diacylglycerol pyrophosphate; DTT; dithiothreitol; 2D-TLC; two-dimensional thin layer chromatography; EGFP; enhanced green fluorescen

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2013 سفارش ترجمه

Molecular cloning and characterization of TNFSF14 (LIGHT) and its receptor TNFRSF14 (HVEM) in guinea pig (Cavia porcellus)

Keywords: LIGHT; lymphotoxin homolog, inducible and competes with HSV glycoprotein D for HveA and is expressed on T lymphocytes; HVEM; herpesvirus entry mediator; LTβR; lymphotoxin β receptor; DcR3; decoy receptor 3; BTLA; B and T lymphocyte attenuator; APC; anti

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2013 سفارش ترجمه

Inhibition of Mycobacterium tuberculosis PknG by non-catalytic rubredoxin domain specific modification: reaction of an electrophilic nitro-fatty acid with the Fe-S center

Keywords: ANS; 8-anilino-1-naphthalenesulfonic acid ammonium salt; BPS; bathophenanthroline disulfonate; ESI; electrospray ionization; IAM; iodoacetamide; IPTG; isopropyl β-D-1-thiogalactopyranoside; LC; liquid chromatography; OA oleic acid; 9-octadecenoic acid; O

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Molecular basis for the catalytic inactivity of a naturally occurring near-null variant of human ALOX15

Keywords: LOXs; lipoxygenases; ALOX; arachidonate lipoxygenase; 15-H(p)ETE; (5Z,8Z,11Z,13E)-15-hydroperoxyeicosa-5,8,11,13-tetraenoic acid; 13-H(p)ODE; (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoic acid; 12-H(p)ETE; (5Z,8Z,10E,14Z)-12-hydroperoxyeicosa-5,8,10,14

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Purification and biochemical analysis of catalytically active human cdc25C dual specificity phosphatase

Keywords: cdc25C; Dual-specificity phosphatases; cdk activation; Purification; 3-OMFP; 3-O-methylfluorescein phosphate; BME; β-mercapto-ethanol; C377S; human cdc25C mutated cysteine 377 serine; cdk; cyclin dependent kinase; DTT; dithiothreitol; IPTG; isopropyl β-

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

cDNA cloning of a snake venom metalloproteinase from the eastern diamondback rattlesnake (Crotalus adamanteus), and the expression of its disintegrin domain with anti-platelet effects

Keywords: Snake venom metalloproteinase; Disintegrin; Crotalus adamanteus; cDNA library; Platelet activity; ACT; activated clotting time; ADP; adenosine diphosphate; cDNA; complementary deoxyribonucleic acid; CR; clot rate; IPTG; isopropyl β-d-thiogalactoside; PCR

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Enhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm

Keywords: Fab; fragment-antigen-binding; Fc; fragment crystallizable; Fd; fragment of antibody consisting of CH1 and VH; ScFv; single-chain variable fragment; VH; variable heavy; VL; variable light; PPIase; peptidyl prolyl cis-trans isomerase; PCR; polymerase chain

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking

Keywords: AR; apelin receptor; AR55; residues 1-55 of AR; BSA; bovine serum albumin; CSI; chemical shift index; DMEM; Dulbecco's modified eagle medium; DPC; dodecylphosphocholine; DPPC; dipalmitoylphosphatidylcholine; DSS; sodium 2,2-dimethyl-2-silapentane-5-sulf

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2013 سفارش ترجمه

Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons

Keywords: RTN; reticulon protein; ER; endoplasmic reticulum; RyR; ryanodine receptor; RHD; reticulon homology domain; CICR; calcium induced calcium release; IPTG; isopropyl-β-d-thiogalactopyranoside; PB; phosphate buffer; DAB; 3,3′-diaminobenzidine tetrahydrochl

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2013 سفارش ترجمه

Biotechnological advances on Penicillin G acylase: Pharmaceutical implications, unique expression mechanism and production strategies

Keywords: 6-APA; 6-aminopenicillianic acid; 7-ACA; 7-aminocephalosporanic acid; 7-ADCA; 7-amino-desacetoxycephalonsporic acid; AA; amino acid; AfPGA; PGA from A. faecalis; βLA; β-lactam antibiotics; BRP; bacteroicin release protein; CRP; cAMP receptor protein; D-

دانلود رایگان متن کامل مقاله ISI 14 صفحه سال انتشار : 2013 سفارش ترجمه

High-level heterologous expression of fungal cytochrome P450s in Escherichia coli

Keywords: Eukaryotic cytochrome P450; High-level expression; Escherichia coli; Large-scale screening; Heterologous expression; Wood-rotting basidiomycetes; EDTA; ethylenediaminetetraacetic acid; HEPES; 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid; IPTG;

دانلود رایگان متن کامل مقاله ISI 38 صفحه سال انتشار : 2013 سفارش ترجمه

Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize

Keywords: Zea mays; Proteolytically active and inactive Deg1 isoforms; Light dependent Deg1-mediated degradation of PSII components in bundle sheath and mesophyll chloroplasts; Red and far red monochromatic light; PSII turnover; BSA; bovine serum albumin; Chl; chlo

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2013 سفارش ترجمه

The thioredoxin system in the dental caries pathogen Streptococcus mutans and the food-industry bacterium Streptococcus thermophilus

Keywords: Thioredoxin system; Streptococcus species; Microaerophiles; Dental pathogen; Food industry bacterium; DTNB; 5,5′-dithiobis-2-nitrobenzoic acid (DTNB); DTT; dithiothreitol; Ea; energy of activation; IPTG; isopropyl-β-thiogalactopyranoside; ROS; reactive

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Expression of recombinant human cathepsin K is enhanced by codon optimization

Keywords: CTSK; cathepsin K; proCTSK; procathepsin K; rproCTSK; recombinant proCTSK; IPTG; isopropyl-β-d-thiogalactopyranoside; PMSF; phenylmethanesulfonylfluoride; IB; inclusion body; GSH; reduced glutathione; GSSG; oxidized glutathione; E-64; 1-[l-N-(trans-epoxy

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2012 سفارش ترجمه

Overexpression of human antiquitin in E. coli: Enzymatic characterization of twelve ALDH7A1 missense mutations associated with pyridoxine-dependent epilepsy

Keywords: AASA; α-aminoadipic semialdehyde; ATQ; antiquitin; HGMD; Human Genome Mutation Database; IPTG; isopropylthiogalactoside; P6C; Δ1-piperideine 6-carboxylate; PDE; pyridoxine dependent epilepsy; PLP; pyridoxal phosphate; Metabolic epilepsy; Pyridoxine; Mis

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2012 سفارش ترجمه

Crystal structure of periplasmic catecholate-siderophore binding protein VctP from Vibrio cholerae at 1.7 Å resolution

Keywords: IPTG; isopropyl β-d-1-thiogalactopyranoside; PMSF; phenyl methane sulfonyl fluoride; r.m.s. deviation; root mean square deviation; PBP; periplasmic binding protein; ABC-transporter; ATP-binding cassette transporter; VctP; Siderophore; Periplasmic binding

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Site-directed mutagenesis and over expression of aroG gene of Escherichia coli K-12

Keywords: PEP; phosphoenolpyruvate; E4P; erythrose 4-phosphate; DAHP; 3-deoxy-d-arabino-heptulosonate-7-phosphate; PCR; polymerase chain reaction; SOE-PCR; splicing overlap extension polymerase chain reaction; LB; Luria Bertani; IPTG; isopropyl-β-d-thiogalactopyra

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

A novel protein Jpk induces bacterial cell death through reactive oxygen species

Keywords: IPTG; isopropyl β-d-thiogalactopyranoside; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; GST; glutathione S-transferase; C.F.U.; colony formation unit; DTT; dithiothreitol; ROS; reactive oxygen species; PSRE; position-specific regu

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Construction of cDNA library and preliminary analysis of expressed sequence tags from tea plant [Camellia sinensis (L) O. Kuntze]

Keywords: %; Percent; °C; Degree; dbEST; database EST; DMSO; Dimethyl sulphoxide; cDNA; Complementary Deoxyribonucleic acid; EST; Expressed Sequence Tag; et al; Et alia; Fig.; Figure; ml; Milliliter; mRNA; Messenger RNA; NCBI; National Center for Biotechnology Inf

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Molecular characterization, tissue distribution and expression analysis of Interleukin-12 receptor β2 chain in sheep

Keywords: APC; antigen-presenting cells; CTL; cytotoxic lymphocytes; FN3; fibronectin type 3; IL-12; Interleukin-12; IL-12R; interleukin (IL)-12 receptor; IL-12Rβ1; interleukin (IL)-12 receptor beta 1; IL-12Rβ2; interleukin (IL)-12 receptor beta 2; IPTG; isopropy

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Effect of periplasmic expression of recombinant mouse interleukin-4 on hydrogen peroxide concentration and catalase activity in Escherichia coli

Keywords: ROS; reactive oxygen species; mIL-4; mouse interleukin-4; MetE; cobalamin-independent methionine synthase; HPI; hydroperoxidase I; HPII; hydroperoxidase II; SOD; superoxide dismutase; OD; optical density; PCR; polymerase chain reaction; IPTG; isopropyl-be

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase

Keywords: CBB; Coomassie Brilliant Blue R250; CDNB; 1-chloro-2,4-dinitrobenzene; ECA; ethacrynic acid; GTX; S-hexyl glutathione; GSH; glutathione; GST; glutathione transferase; 4-HNE; 4-hydroxynonenal; IPTG; isopropyl 1-thio-β-d-galactoside; LB; Luria-Bertani me

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Roles of hinge region, loops 3 and 4 in the activation of Escherichia coli cyclic AMP receptor protein

Keywords: cAMP; 3′,5′-cyclic adenosine monophosphate; PKA; protein kinase A; Epac; exchange protein directly activated by cAMP; CRP; cAMP receptor protein; CNGC; cyclic nucleotide-gated ion channels; WT; wild-type; TEK100; 50 mM Tris 1 mM EDTA and 100 mM KCl

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Crystal and solution structures disclose a putative transient state of mitogen-activated protein kinase kinase 4

Keywords: JNK; c-Jun NH2-terminal kinase; MAP; mitogen-activated protein; MAP2K; MAP kinase kinase; MAP3K; MAP kinase kinase kinase; npMAP2K4; non-phosphorylated MAP2K4; IPTG; isopropyl-β-d-thiogalactopyranoside; ANP; adenylyl-imidodiphosphate; ATP; adenosine trip

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Characterization of human cytomegalovirus UL146 transcripts

Keywords: HCMV; human cytomegalovirus; ORF; open reading frame; HELF; human embryonic lung fibroblast; PFU; plaque forming units; IE; immediate early; X-Gal; 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; IPTG; isopropyl-β-d-thio-galactoside; Cytomegalovirus;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Cloning and characterization of a surface antigen CiSA-32.6 from Cryptocaryon irritans

Keywords: CiSA-32.6 or CiSA-32.6; the gene or protein of surface antigen from Cryptocaryon irritans with a molecular mass of 32.6 kDa; rCiSA-32.6t; recombinant truncated CiSA-32.6; ESTs; expression sequence tags; ORF; open reading frame; IPTG; isopropylthio-β-d-g

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp. NJ-18

Keywords: aa; amino acid residue(s); bp; base pair(s); cDNA; complementary DNA; Ct; threshold cycle; IPTG; isopropyl β-d-thiogalactopyranoside; 5′ RACE; rapid amplification of 5′-cDNA ends; h; hour; kb; kilobase(s); kDa; kilodalton; min; minute; nt; nucleotide

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins

Keywords: Ribotoxin; Cytotoxic protein; Ribosome; Ribonucleases; α-Sarcin; HtA; hirsutellin A; SRL; sarcin-ricin loop; IPTG; isopropyl α-d-thiogalactopyranoside; Tris; tris(hydroxymethyl)aminomethane; Mops; 3-[N-morpholino]propanesulfonic acid; Ni-NTA; Ni2+ nit

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye

Keywords: Rye; Sec2; Secalin; Seed storage protein; GISH; Genomic in situ hybridization; HMW; High-molecular-weight; IPTG; Isopropyl β-Δ-thiogalactopyranoside; SDS-PAGE; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Antimicrobial activity of recombinant Pg-AMP1, a glycine-rich peptide from guava seeds

Keywords: AMPs; antimicrobial peptides; ATCC; American type culture collection; BL21-(DE3); bacterial expression systems; BSA; bovine serum albumin; CFU; colony-forming unit; GRPs; glycine rich proteins; GST; glutathione S-transferase; HDP; host-defense peptide;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA

Keywords: TMG; methyl-1-thio-β-d-galactopyranoside; IPTG; isopropyl β-d-1-thiogalactopyranoside; lac promoter; Gratuitous inducer; Bimodal induction; Single cell studies;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

Expression and one-step purification of a β-galactosidase by fusion with elastin-like polypetides

Keywords: ELPs; elastin like polypeptides; RDL; recursive directional ligation; ITC; inverse transition cycling; IPTG; isopropyl-β-d-thiogalactoside; Trx; thioredoxin; CAT; chloramphenicol acetyltransferase; BFP; blue fluorescent protein; GFP; green fluorescent pr

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه

The N-terminus modulates human Caf1 activity, structural stability and aggregation

Keywords: ANS; 1-anilinonaphtalene-8-sulfonate; CD; circular dichroism; DTT; dithiothreitol; hCaf1; human Caf1; IPTG; isopropyl-1-thio-β-d-galactopyranoside; SDS; sodium dodecyl sulfate; SDS-PAGE; SDS-polyacrylamide gel electrophoresis; SEC; size-exclusion chromat

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2012 سفارش ترجمه