A model for data analysis of microRNA expression in forensic body fluid identification
Keywords: miRNA; Body fluid identification; TaqMan; RT-qPCR; Forensic medicine
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Selection of valid reference genes for expression studies of hepatic cell lines under IFN-α treatment
Keywords: RT-qPCR; geNorm; NormFinder; Reference genes; IFN-α; Huh7; HepG2
Circulating MicroRNA in Digestive Tract Cancers
Keywords: AUC; area under the receiver operating curve; CP; chronic pancreatitis; CRC; colorectal cancer; ESCC; esophageal squamous cell carcinoma; GC; gastric cancer; HCC; hepatocellular cancer; miRNA; microRNA; PC; pancreatic cancer; PCR; polymerase chain reactio
Digital PCR provides absolute quantitation of viral load for an occult RNA virus
Keywords: GBV-C; GB Virus Type C; RT-qPCR; reverse transcriptase quantitative PCR; LNA; locked nucleic acid probes; RT-dPCR; reverse transcriptase digital PCR; CV; coefficient of variation; SEM; standard error of mean; GBV-C; LNA probes; Microfluidics; Occult virus
Investigation of sanguinarine and chelerythrine effects on LPS-induced inflammatory gene expression in THP-1 cell line
Keywords: DMSO; dimethylsulphoxide; FBS; foetal bovine serum; IL; interleukin; LPS; lipopolysaccharide; MCP-1; monocyte chemoattractant protein 1; PBS; phosphate-buffer saline; PCR; polymerase chain reaction; PMA; phorbol myristate acetate; RT-qPCR; reverse transcr
Chlormadinone acetate suppresses prostaglandin biosynthesis in human endometrial explants
Keywords: Chlormadinone acetate; dysmenorrhoea; endometrial explants; prostaglandins; COX-2; RT-qPCR
Differential Expression of Class I Small Leucine-Rich Proteoglycans in an Animal Model of Partial Bladder Outlet Obstruction
Keywords: urinary bladder neck obstruction; fibrosis; inflammation; proteoglycans; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; pBOO; partial bladder outlet obstruction; PBS; phosphate buffered saline; PCR; polymerase chain reaction; RT-qPCR; reverse transcript
Validation of hisH4 and cox5 reference genes for RT-qPCR analysis of gene expression in Aspergillus flavus under aflatoxin conducive and non-conducive conditions
Keywords: Aspergillus flavus; RT-qPCR; Validation of reference genes
Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp. by quantitative real-time RT-PCR
Keywords: Nannochloropsis sp.; Gene expression; Normalization; RT-qPCR; Reference genes
Toll recognition signal activates oenocytoid cell lysis via a crosstalk between plasmatocyte-spreading peptide and eicosanoids in response to a fungal infection
Keywords: ACB; anticoagulant buffer; dsRNA; double-stranded RNA; GBP; growth-blocking peptide; OCL; oenocytoid cell lysis; PG; prostaglandin; PLA2; phospholipase A2; PO; phenoloxidase; PSP; plasmatocyte-spreading peptide; RNAi; RNA interference; RT-qPCR; real time
DNA repair gene expression level in peripheral blood and tumour tissue from non-small cell lung cancer and head and neck squamous cell cancer patients
Keywords: NSCLC; non-small cell lung cancer; HNSCC; squamous cell carcinoma of the head and neck; NER; nucleotide excision repair; RT-qPCR; reverse transcription-quantitative polymerase chain reaction; RQ; relative quantification; Cq; quantification cycle; DNA repa
LRRC3B gene is frequently epigenetically inactivated in several epithelial malignancies and inhibits cell growth and replication
Keywords: Cancer; Tumor suppressor gene; NotI microarrays; Methylation; RT-qPCR
Quantitative real-time RT-PCR assay for research studies on enterovirus infections in the central nervous system
Keywords: EV; enterovirus; IC; internal control; EV-RNA QS; enterovirus-RNA quantitative Standards; RT-qPCR; reverse transcriptase quantitative polymerase chain reaction; Ct; threshold cycle; CSF; cerebrospinal fluid; EU-QCMD; European Union Quality Control for Mol
Identification of reference microRNAs and suitability of archived hemopoietic samples for robust microRNA expression profiling
Keywords: MicroRNAs; RT-qPCR; Blood; Bone marrow; Reference microRNAs; PAXgene⢠blood RNA tube; Long-term storage; Neuroblastoma;
GUSB and ATP2B4 are suitable reference genes for CFTR gene expression data normalization in nasal epithelium cells
Keywords: Cystic fibrosis; CFTR; Gene expression; Nasal epithelium; Reference gene; RT-qPCR;
Critical selection of internal control genes for quantitative real-time RT-PCR studies in lipopolysaccharide-stimulated human THP-1 and K562 cells
Keywords: THP-1 cells; K562 cells; LPS; Internal control genes; RT-qPCR
Analysis of SERPING1 expression on hereditary angioedema patients: Quantitative analysis of full-length and exon 3 splicing variants
Keywords: C1-Inh, C1 inhibitor; HAE, hereditary angioedemaC1 inhibitor; Hereditary angioedema; Alternative splicing; RT-qPCR
Immunoprotective activity of a Salmonid Alphavirus Vaccine: Comparison of the immune responses induced by inactivated whole virus antigen formulations based on CpG class B oligonucleotides and poly I:C alone or combined with an oil adjuvant
Keywords: NAbs; neutralizing antibodies; Ag; antigen; ODNs; oligodeoxynucleotides; TLR; toll-like receptor; APC; antigen-presenting cells; PRRs; pattern recognition receptors; RPP; relative percent protection; HK; head kidney; RT-qPCR; reverse transcriptase quantit
Trichoderma harzianum expressed sequence tags for identification of genes with putative roles in mycoparasitism against Fusarium solani
Keywords: cDNA library; Trichoderma harzianum; Mycoparasitism; Fusarium solani; RT-qPCR
Ozone and aging up-regulate type II metacaspase gene expression and global metacaspase activity in the leaves of field-grown maize (Zea mays L.) plants
Keywords: Tropospheric O3; Type II metacaspases; Zea mays L.; RT-qPCR; Global metacaspase activities
Duplex RT-qPCR for the detection of hepatitis E virus in water, using a process control
Keywords: RT-qPCR; Hepatitis E virus; Murine norovirus; Process control; Water
Trib3 is regulated by IL-3 and affects bone marrow-derived mast cell survival and function
Keywords: ATF; activating transcription factor; BMMCs; bone marrow-derived mast cells; BSA; bovine serum albumin; C/EBP; CCAAT/enhancer binding protein; CHOP; C/EBP homologous protein; DAPI; 4â²,6-diamidino-2-phenylindole; DNP; dinitrophenyl; Epo; erythropoietin;
Comparative procedures for sample processing and quantitative PCR detection of grapevine viruses
Keywords: Grapevine; Virus detection; RNA extraction; RT-qPCR; LDA;
Effect of human TGF-β on the gene expression profile of Schistosoma mansoni adult worms
Keywords: AMPK; 5â²AMP-activated protein kinase; BMP; bone morphogenic protein; CBP; CREB binding protein; eIF; eukaryotic translation initiation factor; Erk; extracellular signal-regulated kinases; GEO; gene expression omnibus; GO; Gene Ontology; hTGF-β; human t
Characterisation of norovirus contamination in an Irish shellfishery using real-time RT-qPCR and sequencing analysis
Keywords: Norovirus; Oysters; Seasonality; Multiple genotypes; RT-qPCR
Comparison of two extraction methods for the detection of hepatitis A virus in semi-dried tomatoes and murine norovirus as a process control by duplex RT-qPCR
Keywords: RT-qPCR; Hepatitis A virus; Murine norovirus; Process control; Semi-dried tomatoes;
Role of P-glycoprotein in emamectin benzoate (SLICE®) resistance in sea lice, Lepeophtheirus salmonis
Keywords: Sea lice (Lepeophtheirus salmonis); P-glycoprotein; Emamectin benzoate; Macrocyclic lactones; Drug resistance; RT-qPCR
Reduction of Na+, K+-ATPase activity and expression in cerebral cortex of glutaryl-CoA dehydrogenase deficient mice: A possible mechanism for brain injury in glutaric aciduria type I
Keywords: CK; creatine kinase; DCIP; dichloroindophenol; DNP; dinitrophenol; EDTA; ethylenediaminetetracetic acid; EGTA; ethylene glycol-bis(2-aminoethylether)-N,N,Nâ²,Nâ²-tetraacetic acid; GA; glutaric acid; GA I; glutaric acidemia type I; GCDH; glutaryl-CoA deh
Differentiation of the virulence potential of Campylobacter jejuni strains by use of gene transcription analysis and a Caco-2 assay
Keywords: Campylobacter; Gene transcription; Environmental stress; Virulence factors; RT-qPCR; Caco-2 cells
Reverse transcription quantitative PCR revealed persistency of thermophilic lactic acid bacteria metabolic activity until the end of the ripening of Emmental cheese
Keywords: Swiss cheese; Ripening; RT-qPCR; Gene expression; Lactic acid bacteria; Starter
Reverse transcriptase real-time PCR for detection and quantification of viable Campylobacter jejuni directly from poultry faecal samples
Keywords: Campylobacter jejuni; RT-qPCR; mRNA; Chicken faeces; Campylobacter survival
Effect of surfactant Tween-80 on sulfur oxidation and expression of sulfur metabolism relevant genes of Acidithiobacillus ferrooxidans
Keywords: Acidithiobacillus ferrooxidans; sulfur metabolism; surfactant Tween-80; RT-qPCR;
AAV6-βARKct gene delivery mediated by molecular cardiac surgery with recirculating delivery (MCARD) in sheep results in robust gene expression and increased adrenergic reserve
Keywords: 2; 17; 29; βAR; β-adrenergic receptor; βARK; β-adrenergic receptor kinase; βARKct; carboxyl terminus of β-adrenergic receptor kinase; cAMP; cyclic adenosine monophosphate; CPB; cardiopulmonary bypass; ESPVR; end-systolic pressure-volume relationsh
B lymphopoiesis is characterized by pre-B cell marker gene expression in fetal cattle and declines in adults
Keywords: BREC; B cell recombination excision circle; HC; heavy chain; IGLL1; immunoglobulin lambda like polypeptide-1; IPP; ileal Peyer's patch; LC; light chain; RT-qPCR; reverse transcription qPCR; VPREB; pre-B lymphocyte gene; B lymphopoiesis; Pre-B cell; Surr
The anti-microbial peptide LL-37 modulates immune responses in the palatine tonsils where it is exclusively expressed by neutrophils and a subset of dendritic cells
Keywords: hCAP-18; Cathelicidin human cationic antimicrobial protein-18; DC; Dendritic cells; TMC; Tonsil mononuclear cells; RT-qPCR; Real-time, reverse transcription quantitative PCR; Palatine tonsils; Anti-microbial peptides; LL-37; Leukocytes; Psoriasis;
Transcriptional response of abscisic acid (ABA) metabolism and transport to cold and heat stress applied at the reproductive stage of development in Arabidopsis thaliana
Keywords: ABA; abscisic acid; ABA-GE; abscisic acid glucose ester; RT-qPCR; real-time quantitative polymerase chain reaction; Arabidopsis; Abscisic acid; Stress; Temperature; Development;
Measurement of the virolysis of human GII.4 norovirus in response to disinfectants and sanitisers
Keywords: Norovirus; NoVs; Calicivirus; RNase; RT-QPCR; Inactivation; Virolysis; Virus infectivity; Virucide;
Host genetic variation in susceptibility to Punta Toro virus
Keywords: dpi; days post infection; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; LD50; 50% lethal dose; PFU; plaque-forming units; PTV; Punta Toro virus; PTV-A; PTV-Adames; PTV-B; PTV-Balliet; RT-qPCR; reverse transcriptase quantitative PCR; RVFV; Rift Valley f
Reference genes for reliable potyvirus quantitation in cassava and analysis of Cassava brown streak virus load in host varieties
Keywords: CBSV; RT-qPCR; Virus diagnosis; Internal controls; Manihot esculenta; Genevestigator; geNORM;
Expression profile of the sphingosine kinase signalling system in the lung of patients with chronic obstructive pulmonary disease
Keywords: COPD; RT-qPCR; Sphingosine 1-phosphate; S1P; Sphingosine kinase activity; S1P receptor 5;
Alteration of the endocannabinoid system in mouse brain during prion disease
Keywords: prion; endocannabinoid; cannabinoid receptor; neurodegenerative disease; brain; mouse; AEA; arachidonoylethanolamide or anandamide; CBD; cannabidiol; CBR; cannabinoid receptor; CJD; Creutzfeldt-Jakob disease; GAPDH; glyceraldehyde-3-phosphate dehydrogenas
Identification of blood microRNAs associated to ParkinsoÅs disease
Keywords: Parkinson's disease; MicroRNA; Neurodegenerative disease; RT-qPCR; Molecular marker;
New RT-qPCR assay for viral nervous necrosis virus detection in sea bass, Dicentrarchus labrax (L.): application and limits for hatcheries sanitary control
Keywords: Dicentrarchus labrax (Sea Bass); Beta-nodavirus; Viral Nervous Necrosis (VNN) virus; RT-qPCR
Msx2 is required for TNF-α-induced canonical Wnt signaling in 3T3-L1 preadipocytes
Keywords: Tumor necrosis factor-α; Msx2; Wnt signaling; β-catenin; Adipocyte; RT-qPCR; reverse transcription-quantitative polymerase chain reaction; LPL; lipoprotein lipase;
Evaluation of reference genes for real-time quantitative PCR in the marine flavobacterium Zobellia galactanivorans
Keywords: Cq, quantification cycle (also known as Ct for threshold cycle); RT-qPCR, quantitative real-time polymerase chain reaction; GR, growth rateAlgae; Marine Bacteroidetes; Marine polysaccharide; Reference genes; RT-qPCR; Zobellia
Critical selection of reliable reference genes for gene expression study in the HepaRG cell line
Keywords: HepaRG; RT-qPCR; Reference gene; Normalization
RT-qPCR based quantitative analysis of gene expression in single bacterial cells
Keywords: Gene expression; Single bacterial cells; RT-qPCR; E. coli; B. subtilis
Estrogen, tamoxifen, and Akt modulate expression of putative housekeeping genes in breast cancer cells
Keywords: Akt; Estrogen; MCF-7; Reference gene; RT-qPCR; Tamoxifen
Characterisation of the FAM69 family of cysteine-rich endoplasmic reticulum proteins
Keywords: ER; endoplasmic reticulum; HA; haemagglutinin; RT-qPCR; Reverse-transcript quantitative polymerase chain reaction; FAM69A; FAM69B; FAM69C; PIP49; Endoplasmic reticulum; Endothelial cells;
Pitfalls of reverse transcription quantitative polymerase chain reaction standardization: Volume-related inhibitors of reverse transcription
Keywords: Reverse transcription; RT-qPCR; mRNA quantification; Standardization; Inhibitor; MIQE guidelines