کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10162348 | 1114327 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and Activity of a New Low-Molecular-Weight Heparin Produced by Enzymatic Ultrafiltration
ترجمه فارسی عنوان
ساختار و فعالیت جدید هپارین با وزن مولکولی پایین تولید شده توسط فوق فرآوری آنزیمی
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کلمات کلیدی
Enzymes - آنزیمUltrafiltration - اولترافیلتراسیونNMR - تشدید مغناطیسی هستهای Surface plasmon resonance - تشدید پلاسمون سطحیPolymeric drugs - داروهای پلیمریStructure-activity relationship - رابطه ساختار-فعالیتAnticoagulant - ضد انعقاد، پادبندMass spectrometry - طیف سنجی جرمیLow molecular weight heparin - هپارین با وزن مولکولی کم Heparin lyase - هپارین لیناز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
چکیده انگلیسی
The standard process for preparing the low-molecular-weight heparin (LMWH) tinzaparin, through the partial enzymatic depolymerization of heparin, results in a reduced yield because of the formation of a high content of undesired disaccharides and tetrasaccharides. An enzymatic ultrafiltration reactor for LMWH preparation was developed to overcome this problem. The behavior, of the heparin oligosaccharides and polysaccharides using various membranes and conditions, was investigated to optimize this reactor. A novel product, LMWH-II, was produced from the controlled depolymerization of heparin using heparin lyase II in this optimized ultrafiltration reactor. Enzymatic ultrafiltration provides easy control and high yields (>80%) of LMWH-II. The molecular weight properties of LMWH-II were similar to other commercial LMWHs. The structure of LMWH-II closely matched heparin's core structural features. Most of the common process artifacts, present in many commercial LWMHs, were eliminated as demonstrated by 1D and 2D nuclear magnetic resonance spectroscopy. The antithrombin III and platelet factor-4 binding affinity of LMWH-II were comparable to commercial LMWHs, as was its in vitro anticoagulant activity. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 5, May 2014, Pages 1375-1383
Journal: Journal of Pharmaceutical Sciences - Volume 103, Issue 5, May 2014, Pages 1375-1383
نویسندگان
Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt,