کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10231543 | 1207 | 2014 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Technical guide for genetic advancement of underdeveloped and intractable Clostridium
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کلمات کلیدی
DCMIEPACEasRNADNA adenine methylaseMOPSFOANGS16S rRNAmRNAHEPESSOE PCRIntron-encoded proteinEPBrRNASMRT sequencing6-methyladenine3-(N-morpholino)propanesulfonic acid - 3- (N-مورفولینو) پروپان سولفونیک اسیدDNA - DNA یا اسید دزوکسی ریبونوکلئیکN-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid - N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acidmessenger RNA - RNA messengerRibosomal RNA - RNA RibosomalAntisense RNA - RNA آنتیسنسsmall non-coding RNA - RNA کوچک غیر کدگذاری5-fluoroorotic acid - اسید 5-فلوروروتیکdeoxyribonucleic acid - اسید deoxyribonucleicribonucleic acid - اسید ریبونوکلئیکRNA - اسید ریبونوکلئیکUltraviolet - اشعه فرابنفشOverexpression - بیش از حد بیانIndustrial biotechnology - بیوتکنولوژی صنعتیNext generation sequencing - توالی نسل بعدیSingle molecule real-time sequencing - توالی واقعی تک سلولی مولکولیTransformation - دگرگونیRam - رمDam - سدSRNA - سرناBiofuels - سوخت های زیستیGC content - محتوای GCRestriction-modification - محدودیت-اصلاحGenetic engineering - مهندسی ژنتیکGene knockout - نابودی ژنnanogram - نانوگرمpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازbase-pair - پایه جفتClostridia - کلسترییدیاClostridium - کلوستریدیوم
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In recent years, the genus Clostridium has risen to the forefront of both medical biotechnology and industrial biotechnology owing to its potential in applications as diverse as anticancer therapy and production of commodity chemicals and biofuels. The prevalence of hyper-virulent strains of C. difficile within medical institutions has also led to a global epidemic that demands a more thorough understanding of clostridial genetics, physiology, and pathogenicity. Unfortunately, Clostridium suffers from a lack of sophisticated genetic tools and techniques which has hindered the biotechnological exploitation of this important bacterial genus. This review provides a comprehensive summary of biotechnological progress made in clostridial genetic tool development, while also aiming to serve as a technical guide for the advancement of underdeveloped clostridial strains, including recalcitrant species, novel environmental samples, and non-type strains. Relevant strain engineering techniques, from genome sequencing and establishment of a gene transfer methodology through to deployment of advanced genome editing procedures, are discussed in detail to provide a blueprint for future clostridial strain construction endeavors. It is expected that a more thorough and rounded-out genetic toolkit available for use in the clostridia will bring about the construction of superior bioprocessing strains and a more complete understanding of clostridial genetics, physiology, and pathogenicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 623-641
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 623-641
نویسندگان
Michael E. Pyne, Mark Bruder, Murray Moo-Young, Duane A. Chung, C. Perry Chou,