کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10586326 | 981389 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pseudoperoxidase investigations of hydroperoxides and inhibitors with human lipoxygenases
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کلمات کلیدی
COXDPPH15-LOX-1DPPPTBA5-LOX12-LOXNDGAcyclooxygenase - آنزیم سیکلواکسیژنازArachidonic acid - اسید آراشیدونیکThiobarbituric acid - اسید تیوباربیتوریکLinoleic acid - اسید لینولئیکnordihydroguaiaretic acid - اسید نوردی هیدروگالیارتیLOX - اکسیژن مایعInhibitor - بازدارندهdiphenyl-1-pyrenylphosphine - دیفنیل-1-پیرینیل فسفینlipoxygenase - لیپواکسیژنازSoybean lipoxygenase-1 - لیپوکسی گیناز سویا 1Hydroperoxide - هیدروپراکسید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pseudoperoxidase investigations of hydroperoxides and inhibitors with human lipoxygenases Pseudoperoxidase investigations of hydroperoxides and inhibitors with human lipoxygenases](/preview/png/10586326.png)
چکیده انگلیسی
Understanding the mode of action for lipoxygenase (LOX) inhibitors is critical to determining their efficacy in the cell. The pseudoperoxidase assay is an important tool for establishing if a LOX inhibitor is reductive in nature, however, there have been difficulties identifying the proper conditions for each of the many human LOX isozymes. In the current paper, both the 234Â nM decomposition (UV) and iron-xylenol orange (XO) assays are shown to be effective methods of detecting pseudoperoxidase activity for 5-LOX, 12-LOX, 15-LOX-1 and 15-LOX-2, but only if 13-(S)-HPODE is used as the hydroperoxide substrate. The AA products, 12-(S)-HPETE and 15-(S)-HPETE, are not consistent hydroperoxide substrates since they undergo a competing transformation to the di-HETE products. Utilizing the above conditions, the selective 12-LOX and 15-LOX-1 inhibitors, probes for diabetes, stroke and asthma, are characterized for their inhibitory nature. Interestingly, ascorbic acid also supports the pseudoperoxidase assay, suggesting that it may have a role in maintaining the inactive ferrous form of LOX in the cell. In addition, it is observed that nordihydroguaiaretic acid (NDGA), a known reductive LOX inhibitor, appears to generate radical species during the pseudoperoxidase assay, which are potent inhibitors against the human LOX isozymes, producing a negative pseudoperoxidase result. Therefore, inhibitors that do not support the pseudoperoxidase assay with the human LOX isozymes, should also be investigated for rapid inactivation, to clarify the negative pseudoperoxidase result.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 13, 1 July 2013, Pages 3894-3899
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 13, 1 July 2013, Pages 3894-3899
نویسندگان
Eric K. Hoobler, Charles Holz, Theodore R. Holman,