کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738345 | 1046702 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC/ESR/MS study of pH-dependent radical generation from 15-LOX-catalyzed DPA peroxidation
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کلمات کلیدی
DPAα-(4-pyridyl-1-oxide)-N-tert-butyl nitroneHDPAPOBNeXtracted Ion CurrentTICMRMACNESRESR spin trapping - ESR تله اسپینLC/MS - LC / MSAcetonitrile - استونیتریلdocosapentaenoic acid - اسید داکوزاپنتانوئیکPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعLOX - اکسیژن مایعtotal ion current - جریان یونی کلFree radicals - رادیکال آزادElectron spin resonance - رزونانس اسپین الکترونRetention time - زمان بازداریMass spectrometry - طیف سنجی جرمیlipoxygenase - لیپواکسیژنازEIC - مهندسانMultiple-reaction monitoring - نظارت چند واکنشhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Docosapentaenoic acid (DPA) is a unique fatty acid that exists in two isomeric forms (n-3 and n-6), which differ in their physiological behaviors. DPA can undergo free radical-mediated peroxidation via lipoxygenase (LOX). 15-LOX, one of the LOX isomers, has received much attention in cancer research because of its very different expression level in normal tissues compared to tumors and some bioactive fatty acid metabolites modulating the tumorigenic pathways in cancer. However, the mechanism linking 15-LOX, DPA metabolites, and their bioactivities is still unclear, and the free radicals generated in DPA peroxidation have never been characterized. In this study, we have studied radicals formed from both soybean and human cellular (PC3-15LOS cells) 15-LOX-catalyzed peroxidation of DPAs at various pH's using a combination of LC/ESR/MS with the spin trapping technique. We observed a total of three carbon-centered radicals formed in 15-LOX-DPA (n-3) stemming from its 7-, 17-, and 20-hydroperoxides, whereas only one formed from 17-hydroperoxide in DPA (n-6). A change in the reaction pH from 8.5 (15-LOX enzyme optimum) to 7.4 (physiological) and to 6.5 (tumor, acidic) not only decreased the total radical formation but also altered the preferred site of oxygenation. This pH-dependent alteration of radical formation and oxygenation pattern may have significant implications and provide a basis for our ongoing investigations of LOXs as well as fatty acids in cancer biology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 51, Issue 7, 1 October 2011, Pages 1461-1470
Journal: Free Radical Biology and Medicine - Volume 51, Issue 7, 1 October 2011, Pages 1461-1470
نویسندگان
Preeti Purwaha, Yan Gu, Uddhav Kelavkar, Jing X. Kang, Benedict Law, Erxi Wu, Steven Y. Qian,