کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738438 | 1046705 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of free radicals formed from COX-catalyzed DGLA peroxidation
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کلمات کلیدی
COXPOBNeXtracted Ion CurrentESRDGLATICESR spin trapping - ESR تله اسپینα-(4-pyridyl-1-oxide)-N-tert-butylnitrone - α- (4-پیریدیل-1-اکسید) -N-tert-butylnitronecyclooxygenase - آنزیم سیکلواکسیژنازdihomo-γ-linolenic acid - اسید dihomo-γ-linolenicArachidonic acid - اسید آراشیدونیکtotal ion current - جریان یونی کلFree radicals - رادیکال آزادElectron spin resonance - رزونانس اسپین الکترونRetention time - زمان بازداریMass spectrometry - طیف سنجی جرمیEIC - مهندسانprostaglandin - پروستاگلاندینهاhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Like arachidonic acid (AA), dihomo-γ-linolenic acid (DGLA) is a 20-carbon Ï-6 polyunsaturated fatty acid and a substrate of cyclooxygenase (COX). Through free radical reactions, COX metabolizes DGLA and AA to form well-known bioactive metabolites, namely, the 1 and 2 series of prostaglandins (PGs1 and PGs2), respectively. Unlike PGs2, which are viewed as proinflammatory, PGs1 possess anti-inflammatory and anticancer activities. However, the mechanisms linking the PGs to their bioactivities are still unclear, and radicals generated in COX-DGLA have not been detected. To better understand PG biology and determine whether different reactions occur in COX-DGLA and COX-AA, we have used LC/ESR/MS with a spin trap, α-(4-pyridyl-1-oxide)-N-tert-butyl nitrone (POBN), to characterize the carbon-centered radicals formed from COX-DGLA in vitro, including cellular peroxidation. A total of five types of DGLA-derived radicals were characterized as POBN adducts: m/z 266, m/z 296, and m/z 550 (same as or similar to COX-AA) and m/z 324 and m/z 354 (exclusively from COX-DGLA). Our results suggest that C-15 oxygenation to form PGGs occurs in both COX-DGLA and COX-AA; however, C-8 oxygenation occurs exclusively in COX-DGLA. This new finding will be further investigated for its association with various bioactivities of PGs, with potential implications for inflammatory diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 9, 1 May 2011, Pages 1163-1170
Journal: Free Radical Biology and Medicine - Volume 50, Issue 9, 1 May 2011, Pages 1163-1170
نویسندگان
Ying Xiao, Yan Gu, Preeti Purwaha, Kunyi Ni, Benedict Law, Sanku Mallik, Steven Y. Qian,