کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739382 | 1046873 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modulation of DNA-dependent protein kinase activity in chlorambucil-treated cells
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کلمات کلیدی
NHEJDNA-PKBSOH2DCF-DADSBsN-acetyl-l-cysteineNBTNACBHADNA-PKcsDNA double-strand breaks - DNA دو رشته شکستهROS - ROSnitroblue tetrazolium - tetrazolium nitroblueGlucose oxidase - آنزیم گلوکزاکسیدازOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادComet assay - روش کامت یا روش سنجش ستاره دنبالهدار DNA-dependent protein kinase catalytic subunit - وابسته به DNA وابسته به پروتئین کیناز کاتالیزوریNonhomologous end joining - پیوستن به انتهای غیرخطیCbl - کلمReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
DNA-dependent protein kinase (DNA-PK) is activated in a two-step process whereby the Ku heterodimer first binds to the DNA double-strand breaks (dsbs) and then the DNA-PK catalytic subunit (cs) is recruited to form a repair complex. Oxidative stress is simultaneously generated along with DNA damage by ionizing radiation or chemotherapeutic agents whose impact on the DNA-PK activity has not previously been investigated. Here we show that the DNA damage-induced kinase activity of DNA-PK was modulated by oxidative stress, which was induced along with DNA dsbs in chlorambucil (Cbl)-exposed cells. Pretreatment with the antioxidants, 2(3)-t-butyl-4-hydroxyanisole or N-acetyl-l-cysteine enhanced the amount of DNA-PKcs phosphorylated at threonine 2609 (DNA-PKpThr2609) at the DNA dsbs and DNA-PK activity. Conversely, oxidative stress induced by l-buthionine (SR)-sulfoximine or glucose oxidase decreased the DNA-PK activity in Cbl-exposed cells. In addition, DNA-PKpThr2609 was poorly detectable at the site of DNA dsbs, as shown by colocalization to DNA-end-binding pH2AX or p53BP1. There was no change in the protein levels of DNA-PKcs, Ku70, or Ku86. Data from these studies provide the first evidence that oxidative stress effects posttranslational modification and assembly of DNA-PK complex at DNA dsbs, and thereby repair of DNA dsbs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 12, 15 December 2005, Pages 1650-1659
Journal: Free Radical Biology and Medicine - Volume 39, Issue 12, 15 December 2005, Pages 1650-1659
نویسندگان
Attila Bacsi, Subbaraj Kannan, Myung-Soog Lee, Tapas K. Hazra, Istvan Boldogh,