کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749286 | 1050287 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histone acetyltransferase PCAF is involved in transactivation of Bcl-6 and Pax5 genes in immature B cells
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کلمات کلیدی
PAX5EBF1H3K9BACH2XBP-1Bcl-6PU.1BLIMP-1PCAFMITFB lymphocyte-induced maturation protein-1E2AIRF-4HDACDT40 - dt40Epigenetics - اپی ژنتیکchromatin immunoprecipitation - ایمن سازی کروماتینB cell development - توسعه سلول BX-box binding protein-1 - جعبه اتصال پروتئین -1P300/CBP-associated factor - عامل P300 / CBP مرتبط استMicrophthalmia-associated transcription factor - فاکتور رونویسی مرتبط با میکرو فتالمیGene targeting - هدف ژنHistone acetyltransferase - هیستون استیل ترانسفرازhistone deacetylase - هیستون داستیلازCHiP - چیپHAT - کلاه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Histone acetyltransferase p300/CBP-associated factor (PCAF) belonging to GCN5 family regulates various epigenetic events for transcriptional regulation through alterations in the chromatin structure. During normal development of B cells, gene expressions of numerous transcription factors are strictly regulated by epigenetic mechanisms including histone acetylation and deacetylation to complete their development pathways. Here, by analyzing PCAF-deficient DT40 mutants, ÎPCAF, we report that PCAF takes part in transcriptional activation of B cell lymphoma-6 (Bcl-6) and Paired box gene 5 (Pax5), which are essential transcription factors for normal development of B cells. PCAF-deficiency caused drastic decrease in mRNA levels of Bcl-6 and Pax5, and remarkable increase in that of B lymphocyte-induced maturation protein-1 (Blimp-1). In addition, chromatin immunoprecipitation assay showed that PCAF-deficiency caused remarkable decrease in acetylation levels of both H3K9 and H3K14 residues within chromatin surrounding the 5'-flanking regions of Bcl-6 and Pax5 genes in vivo, suggesting that their gene expressions may be regulated by PCAF. These results revealed that PCAF is involved in transactivation of Bcl-6 and Pax5 genes, resulting in down-regulation of Blimp-1 gene expression, and plays a key role in epigenetic regulation of B cell development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 3, 20 November 2015, Pages 509-513
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 3, 20 November 2015, Pages 509-513
نویسندگان
Hidehiko Kikuchi, Masami Nakayama, Futoshi Kuribayashi, Hitomi Mimuro, Shinobu Imajoh-Ohmi, Hideki Nishitoh, Tatsuo Nakayama,