کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754589 | 1050357 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mitochondrial tRNA cleavage by tRNA-targeting ribonuclease causes mitochondrial dysfunction observed in mitochondrial disease
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کلمات کلیدی
COXMTSMELASMERRFZFNMitochondrial tRNA - tRNA میتوکندریMitochondrial disease - بیماری میتوکندریاییTranscription activator-like effector nuclease - رونویسی مانند actuator nucleaseribonuclease - ریبونوکلئازTALEN - زبان هاcytochrome c oxidase - سیتوکروم سی اکسیدازMyoclonic epilepsy with ragged-red fibers - صرع میوکلونیک با الیاف سرخ شدهzinc finger nuclease - نیکلاز انگشت روی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mitochondrial DNA (mtDNA) is a genome possessed by mitochondria. Since reactive oxygen species (ROS) are generated during aerobic respiration in mitochondria, mtDNA is commonly exposed to the risk of DNA damage. Mitochondrial disease is caused by mitochondrial dysfunction, and mutations or deletions on mitochondrial tRNA (mt tRNA) genes are often observed in mtDNA of patients with the disease. Hence, the correlation between mt tRNA activity and mitochondrial dysfunction has been assessed. Then, cybrid cells, which are constructed by the fusion of an enucleated cell harboring altered mtDNA with a Ï0 cell, have long been used for the analysis due to difficulty in mtDNA manipulation. Here, we propose a new method that involves mt tRNA cleavage by a bacterial tRNA-specific ribonuclease. The ribonuclease tagged with a mitochondrial-targeting sequence (MTS) was successfully translocated to the mitochondrial matrix. Additionally, mt tRNA cleavage, which resulted in the decrease of cytochrome c oxidase (COX) activity, was observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 1, 15 August 2014, Pages 131-136
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 1, 15 August 2014, Pages 131-136
نویسندگان
Tetsuhiro Ogawa, Ayano Shimizu, Kazutoshi Takahashi, Makoto Hidaka, Haruhiko Masaki,