کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754640 | 1050359 | 2014 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemical chaperones reduce ionizing radiation-induced endoplasmic reticulum stress and cell death in IEC-6 cells
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کلمات کلیدی
ATF4PBATUDCAeIF2αUPRSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAEndoplasmic reticulum stress - استرس شبکه آندوپلاسمی4-phenylbutyric acid - اسید 4-فنیل بوتیریکTauroursodeoxycholic acid - اسید تاورورسید سدیم اسیدionizing radiation - تابش یوننده یا پرتوهای یونیزانChemical chaperone - شاپرئین شیمیendoplasmic reticulum - شبکه آندوپلاسمی eukaryotic translation initiation factor 2α - عامل آغازگر ترجمه یوکاریوتی 2αactivating transcription factor 4 - فعال کردن عامل رونویسی 4Unfolded protein response - پاسخ پروتئین آشکارPERK - پرک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Radiotherapy, which is one of the most effective approaches to the treatment of various cancers, plays an important role in malignant cell eradication in the pelvic area and abdomen. However, it also generates some degree of intestinal injury. Apoptosis in the intestinal epithelium is the primary pathological factor that initiates radiation-induced intestinal injury, but the mechanism by which ionizing radiation (IR) induces apoptosis in the intestinal epithelium is not clearly understood. Recently, IR has been shown to induce endoplasmic reticulum (ER) stress, thereby activating the unfolded protein response (UPR) signaling pathway in intestinal epithelial cells. However, the consequences of the IR-induced activation of the UPR signaling pathway on radiosensitivity in intestinal epithelial cells remain to be determined. In this study, we investigated the role of ER stress responses in IR-induced intestinal epithelial cell death. We show that chemical ER stress inducers, such as tunicamycin or thapsigargin, enhanced IR-induced caspase 3 activation and DNA fragmentation in intestinal epithelial cells. Knockdown of Xbp1 or Atf6 with small interfering RNA inhibited IR-induced caspase 3 activation. Treatment with chemical chaperones prevented ER stress and subsequent apoptosis in IR-exposed intestinal epithelial cells. Our results suggest a pro-apoptotic role of ER stress in IR-exposed intestinal epithelial cells. Furthermore, inhibiting ER stress may be an effective strategy to prevent IR-induced intestinal injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 2, 25 July 2014, Pages 1005-1009
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 2, 25 July 2014, Pages 1005-1009
نویسندگان
Eun Sang Lee, Hae-June Lee, Yoon-Jin Lee, Jae-Hoon Jeong, Seongman Kang, Young-Bin Lim,