کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755350 | 1050372 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell cycle-dependent regulation of Aurora kinase B mRNA by the Microprocessor complex
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کلمات کلیدی
TDFRT-PCRCLIP-seqAurora kinase BUTRhESC - hescSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAHuman embryonic stem cell - سلول بنیادی جنینی انسانuntranslated region - منطقه غیر ترجمهMitosis - میتوزMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسCell cycle - چرخه سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Aurora kinase B regulates the segregation of chromosomes and the spindle checkpoint during mitosis. In this study, we showed that the Microprocessor complex, which is responsible for the processing of the primary transcripts during the generation of microRNAs, destabilizes the mRNA of Aurora kinase B in human cells. The Microprocessor-mediated cleavage kept Aurora kinase B at a low level and prevented premature entrance into mitosis. The cleavage was reduced during mitosis leading to the accumulation of Aurora kinase B mRNA and protein. In addition to Aurora kinase B mRNA, the processing of other primary transcripts of miRNAs were also decreased during mitosis. We found that the cleavage was dependent on an RNA helicase, DDX5, and the association of DDX5 and DDX17 with the Microprocessor was reduced during mitosis. Thus, we propose a novel mechanism by which the Microprocessor complex regulates stability of Aurora kinase B mRNA and cell cycle progression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 1, 28 March 2014, Pages 241-247
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 1, 28 March 2014, Pages 241-247
نویسندگان
Eunsun Jung, Youngmo Seong, Jae Hong Seo, Young-Soo Kwon, Hoseok Song,