کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756938 | 1050389 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ERK2 small interfering RNAs prevent epidural fibrosis via the efficient inhibition of collagen expression and inflammation in laminectomy rats
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کلمات کلیدی
TGF-β1Hydroxyproline contentFBSSIL6SEPsERK2siRNAsGFPHPCDMEMRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsmall interfering RNAs - RNA های تداخل کوچکsiRNA - siRNAinterleukin 6 - اینترلوکین 6Transforming growth factor-β1 - تبدیل فاکتور رشد β1Failed back surgery syndrome - سندروم جراحی شکست خوردهEpidural fibrosis - فیبروز اپیدورالLaminectomy - لامینکتومیDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoLentivirus - ویروس آهستهگستر، لنتی ویروسgreen fluorescent protein - پروتئین فلورسنت سبزAdhesion - چسبندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: ERK2 small interfering RNAs prevent epidural fibrosis via the efficient inhibition of collagen expression and inflammation in laminectomy rats ERK2 small interfering RNAs prevent epidural fibrosis via the efficient inhibition of collagen expression and inflammation in laminectomy rats](/preview/png/10756938.png)
چکیده انگلیسی
Laminectomy is a widely accepted treatment for lumbar disorders. Epidural Fibrosis (EF) is a common post-laminectomy or post-discectomy complication, which is thought to cause recurrent pain. RNA interference (RNAi) is a process by which double-stranded RNA triggers the destruction of mRNAs sharing the same sequence. Previously, extra-cellular signal-regulated kinase (ERK) 2 plays crucial roles in suppressing the collagen expression. To investigate the effects of lentiviral ERK2 siRNA on the prevention of post-laminectomy EF formation in a rat model, a controlled double-blinded study was conducted in 75 healthy adult Wistar rats that underwent laminectomy. They were divided randomly into 3 groups according to the treatment method: (1) control group; (2) ERK scrRNA group; (3) ERK siRNA group. All rats were euthanized humanely 4Â weeks post-laminectomy. The hydroxyproline content, Rydell score, vimentin cells density, fibroblasts density, inflammatory cells density and inflammatory factors expressions were performed. The hydroxyproline content, Rydell score, vimentin cells density, fibroblasts density, inflammatory cells density and inflammatory factors expressions all suggested better results in ERK siRNA group than other two groups. None of the rats expired and no obvious adverse effects were observed. Local delivery of a lentiviral siRNA targeting ERK2 can prevent epidural scar adhesion in post-laminectomy rat via inhibiting collagen expression and inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 444, Issue 3, 14 February 2014, Pages 395-400
Journal: Biochemical and Biophysical Research Communications - Volume 444, Issue 3, 14 February 2014, Pages 395-400
نویسندگان
Chao Zhang, Xiaohong Kong, Chang Liu, Zhipin Liang, Huabing Zhao, Wei Tong, Guangzhi Ning, Wenyuan Shen, Liwei Yao, Shiqing Feng,