کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10758145 | 1050404 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calumenin has a role in the alleviation of ER stress in neonatal rat cardiomyocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ERADERp57Ad-LacZtunicamycinIREPBAChaperoneThapsigarginGRPUPReIF2ATFC/EBP-homologous protein - C / EBP-homologous proteinMOI - MESDS–PAGE - SDS-PAGE4-phenylbutyric acid - اسید 4-فنیل بوتیریکSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتifu - اگرER-Associated Degradation - تخریب ER-AssociatedTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهTUNEL - تونلCHOP - تکه کردنApoptosis - خزان یاختهایSarcoplasmic reticulum - رتیکولوم سارکوپلاسمیکeukaryotic initiation factor 2 - عامل آغاز کننده یوکاریوتی 2activating transcription factor - فعال کردن عامل رونویسیinfectious unit - واحد عفونیUnfolded protein response - پاسخ پروتئین آشکارprotein kinase RNA-like endoplasmic reticulum kinase - پروتئین کیناز RNA مانند اندوپلاسمی رتیکولوم کینازPERK - پرکmultiplicity of infection - چندین عفونتneonatal rat ventricular cardiomyocytes - کاردیومیوسیت های بطنی ریه نوزادی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Disturbance of endoplasmic reticulum (ER) homeostasis causes ER stress (ERS), and triggers the unfolded protein response (UPR) that consequently reduces accumulation of unfolded proteins by increasing the quantity of ER chaperones. Calumenin, a Ca2+-binding protein with multiple EF hand motifs, which is located in the ER/SR, is highly expressed during the early developmental stage of the heart, similar to other ER-resident chaperones. The aim of this study was to investigate the functional role of calumenin during ERS in the heart. Like other chaperones (e.g., GRP94 and GRP78), calumenin expression was highly upregulated during ERS induced by 10 μg/ml tunicamycin, but attenuated in the presence of 500 μM PBA, the chemical chaperone in neonatal rat ventricular cardiomyocytes (NRVCs). Upon 7.5-fold overexpression of calumenin using a recombinant adenovirus system, the expression levels of ERS markers (GRP78, p-PERK, and p-elF2α) and ER-initiated apoptosis markers (CHOP and p-JNK) were reduced, whereas the survival protein BCL-2 was upregulated during ERS compared to the control. Evaluation of cell viability by TUNEL assay showed that apoptosis was also significantly reduced by calumenin overexpression in ERS-induced cells. Taken together, our results suggest that calumenin plays an essential role in the alleviation of ERS in neonatal rat cardiomyocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 439, Issue 3, 27 September 2013, Pages 327-332
Journal: Biochemical and Biophysical Research Communications - Volume 439, Issue 3, 27 September 2013, Pages 327-332
نویسندگان
Joo Hee Lee, Eun Jeong Kwon, Do Han Kim,