کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803030 | 1055764 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNMT1 interacts with the developmental transcriptional repressor HESX1
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کلمات کلیدی
IVTRNF2GSTHRPPCPPFADNA methyltransferase 1EZH2homeoboxTLE1Dnmt1N-CoR - N-CORamino acid - آمینو اسیدSepto-optic dysplasia - دیسپلازی سپتو-اپتیکSOD - سدRepression - سرکوبES cells - سلول ESEmbryonic stem cells - سلولهای بنیادی جنینیPituitary - غدهٔ زیرمغزی یا هیپوفیزDNA methylation - متیلاسیون DNAMouse - موشparaformaldehyde - پارافرمالدهیدhorse radish peroxidase - پراکسیداز تربچه اسبforebrain - پیشانیpolycomb group - گروه پلی کامبglutathione-S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hesx1 is a highly conserved homeobox gene present in vertebrates, but absent from invertebrates. Gene targeting experiments in mice have shown that this transcriptional repressor is required for normal forebrain and pituitary development. In humans, mutations in HESX1 impairing either its repressing activity or DNA binding properties lead to a comparable phenotype to that observed in Hesx1 deficient mice. In an attempt to gain insights into the molecular function of HESX1, we have performed a yeast two-hybrid screen and identified DNA methyltransferase 1 (DNMT1) as a HESX1 binding protein. We show that Dnmt1 is co-expressed with Hesx1 within the anterior forebrain and in the developing Rathke's pouch. Mapping of the interacting regions indicates that the entire HESX1 protein is required to establish binding to a portion of the N-terminus of DNMT1 and its catalytic domain in the C-terminus. The HESX1-DNMT1 complexes can be immunoprecipitated in cells and co-localise in the nucleus. These results establish a link between HESX1 and DNMT1 and suggest a novel mechanism for the repressing properties of HESX1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 1, January 2008, Pages 131-143
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 1, January 2008, Pages 131-143
نویسندگان
Ezat Sajedi, Carles Gaston-Massuet, Cynthia L. Andoniadou, Massimo Signore, Paul J. Hurd, Mehul Dattani, Juan Pedro Martinez-Barbera,