کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815810 | 1058505 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lithium induces c-Ret expression in mouse inner medullary collecting duct cells
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کلمات کلیدی
TSAGDNF family receptor alphaGFRαS6KTSC2TCF7L2HGFHIF6-bromoindirubin-3′-oximeLiClGDNFGSK-3βNaClmTORHDACLithium - لیتیمS6 kinase - S6 کینازTCF/LEF - TCF / LEFBIO - بیوTrichostatin A - تریکوستاتین Ac-Ret - ج-بازنشستهureteric bud - جوانه شکمبهRET - حقRearranged during transfection - در ترانسفکشن دوباره تنظیم شده استRapamycin - راپامایسینSodium chloride - سدیم کلریدHepatocyte growth factor - عامل رشد هپاتوسیتHypoxia induced factor - عامل ناشی از هیپوکسیGlial cell line-derived neurotrophic factor - فاکتور نوروتروفی مشتق از سلول گلیاییLi+ - لی +lithium chloride - لیتیم کلریدmetanephric mesenchyme - مزانشیم metanephricmammalian target of rapamycin - هدف پستانداران رپامایسینhistone deacetylase - هیستون داستیلازGlycogen synthase kinase 3β - گلیکوزین سنتاز کیناز 3β
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We found in our present study that lithium (Li+) induced the expression of endogenous c-Ret, a tyrosine kinase receptor, in murine inner medullary collecting duct (mIMCD-3) cells. Delineation of the promoter region required for the effect of Li+ identified a positive regulatory element within 180 bp upstream of the transcription initiation site. This region contained three putative GC-rich Sp1 binding sites found to be essential for c-Ret induction by Li+. The effect of Li+ was mediated through glycogen synthase kinase 3β (GSK-3β) inhibition, although there was no biding site for T cell factor/lymphoid enhancer factor (TCF/LEF) in the 180 bp. We found that Li+ activated the mammalian target of rapamycin (mTOR) pathway via GSK-3β in these cells, and the effect of Li+ to induce c-Ret was amenable to the inhibitory effect of the mTOR inhibitor, rapamycin. We also found that alterations in both cellular β-catenin levels and mTOR activities affected the effect of Li+ on c-Ret transcription in a cooperative manner. In summary, our results show that Li+ can induce c-Ret expression in mIMCD-3 cells through both β-catenin- and mTOR-dependent pathways downstream of GSK-3β inhibition, which act synergistically on the GC-rich Sp1 binding elements in the promoter region.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 23, Issue 2, February 2011, Pages 371-379
Journal: Cellular Signalling - Volume 23, Issue 2, February 2011, Pages 371-379
نویسندگان
Nobuhiko Kojima, Hiroshi Saito, Masaki Nishikawa, Shunsuke Yuri, Oak Don Jo, Phuong-Chi Pham, Naomi Yanagawa, Norimoto Yanagawa,