In vitro isolation, elicitation of psoralen in callus cultures of Psoralea corylifolia and cloning of psoralen synthase gene

► Isolation of the psoralen bioactive anticancerous compound from callus cultures of Psoralea corylifolia has been reported for the first time. ► Augmentation of callus with elicitors such as yeast extract, cinnamic acid and sucrose significantly enhanced the synthesis of psoralen. ► Putative psoralen synthase gene from P. corylifolia has been isolated and cloned which show significant homology with psoralen synthase of Ammi majus.