کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903727 | 1086518 | 2016 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short-term preconditioning enhances the therapeutic potential of adipose-derived stromal/stem cell-conditioned medium in cisplatin-induced acute kidney injury
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کلمات کلیدی
HGFHMGB1KIM-1i.p.MASCSHYPMesenchymal stromal/stem cellsBM-MSCsHypoxic environmentbFGFMSCsAKIPCMMMP12IGFTNFαASCsacute kidney injury - آسیب حاد کلیهinsulin-like growth factor-1 - انسولین مانند عامل رشد 1kidney regeneration - بازسازی کلیهtumor necrosis factor-alpha - تومور نکروز عامل آلفاintraperitoneal - داخل صفاقیadipose-derived stromal/stem cells - سلول های استروما / سلول های بنیادی حاوی چربیConditioned medium - شرایط محیطیHepatocyte growth factor - عامل رشد هپاتوسیتVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)basic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهneutrophil gelatinase-associated lipocalin - لیوتوکالین مرتبط با نوتروفیل ژلاتینازMatrix metalloproteinase-12 - ماتریکس متالوپروتئیناز-12bone marrow - مغز استخوانKidney injury molecule-1 - مولکول آسیب کلیه-1Renal failure - نارسایی کلیهHypoxia - هیپوکسیPCR - واکنش زنجیرهٔ پلیمرازPreconditioning - پیش شرط بندیserum creatinine - کراتینین سرم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The development of new strategies to preserve renal function after acute kidney injury (AKI) is necessary due to limited clinical intervention options. The organ-protective effects of mesenchymal stromal/stem cells (MSCs) and their conditioned medium (CM) have been investigated demonstrating that both separately promoted tubular recovery and ameliorated the outcome of AKI. Nevertheless, strategies to optimise the regenerative potential of both are highly needed. Here we investigated the effects of CM from adipose-derived MSCs (ASCs) preincubated in a hypoxic environment (Hyp). Protective factors were investigated by PCR analysis and a protein array in vitro. The expression of 64 of the 308 proteins assayed was found to be more than two-fold increased after Hyp. CM of Hyp-pretreated ASCs (pCM) was used to enhance regeneration in a mouse model of cisplatin-induced AKI (cisAKI). Renal function was assessed by measurements of markers for AKI and serum cytokine levels. The pCM significantly ameliorated serum creatinine and neutrophil gelatinase-associated lipocalin values, and also the levels of inflammatory cytokines IL-1β and IL-6 in the serum of mice with AKI. Our work clearly showed that a Hyp preconditioning significantly increases the release of protective factors in ASCs and enhances the therapeutic effects of CM in cisAKI in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 342, Issue 2, 15 March 2016, Pages 175-183
Journal: Experimental Cell Research - Volume 342, Issue 2, 15 March 2016, Pages 175-183
نویسندگان
Jürgen M. Overath, Stefan Gauer, Nicholas Obermüller, Ralf Schubert, Richard Schäfer, Helmut Geiger, Patrick C. Baer,