کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903804 | 1086527 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The acquired radioresistance in HeLa cells under conditions mimicking hypoxia was attenuated by a decreased expression of HIF subunit genes induced by RNA interference
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کلمات کلیدی
Oxygen dependent degradation domainpVHLHREPDGFHIFCXCR4shRNAGAPDHCSCPer-Arnt-Sim - per-arnt-simshort hairpin RNA - RNA موی سر کوتاهLET - اجازه دهیدLinear Energy Transfer - انتقال انرژی خطیHAF - تابستانcancer stem cell - سلولهای بنیادی سرطانیhypoxia response element - عنصر پاسخ هیپوکسیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)platelet-derived growth factor - فاکتور رشد حاصل از پلاکتHypoxia Inducible Factor - فاکتور قابل تشخیص هیپوکسیODD - فردMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAPAS - نهHypoxia - هیپوکسیRadiation - پرتوCobalt chloride - کلرید کبالتglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The cancer cells residing in the hypoxic layer are resistant to radiation and these are ones responsible for cancer recurrence after radiation therapy. One of the reasons why hypoxic cancer cells acquire radioresistance may be attributable to changes in the gene expression profile by the activation of hypoxia inducible factors (HIFs). However, the details underlying this process remain unknown. In this study, we investigated the effects of knockdown of HIF subunit genes to elucidate how HIF subunit genes may be involved in the radioresistance acquired by HeLa cells following exposure to a hypoxia mimic. Interestingly, HIF-1α and HIF-2α seemed mutually complementary for each other when either of them was suppressed. We thus suppressed the expression of both genes simultaneously. To do this, we developed a short hairpin RNA (shRNA) targeting a high homology region between HIF-1α and HIF-2α. It was shown that the expression of the shRNA effectively suppressed the acquisition of radioresistance following the hypoxia mimic. Moreover, it was confirmed that suppression of both subunits resulted in the downregulation of stem cell markers and the suppression of spheroid formation during the hypoxia mimicking-conditions. This shRNA-mediated knockdown method targeting a common region shared by a family of genes may offer a new candidate cancer treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 333, Issue 2, 1 May 2015, Pages 249-260
Journal: Experimental Cell Research - Volume 333, Issue 2, 1 May 2015, Pages 249-260
نویسندگان
Nobutaka Doi, Ryohei Ogawa, Zheng-Guo Cui, Akihiro Morii, Akihiko Watanabe, Shinji Kanayama, Yuko Yoneda, Takashi Kondo,