کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903818 | 1086529 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ascribing novel functions to the sarcomeric protein, myosin binding protein H (MyBPH) in cardiac sarcomere contraction
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
cMyBPCMEL1ADE2HIS3FAF1ACTC1myosin binding protein HMYBPHFBN1ABAMYH7LVHFLNCHCMautophagy-lysosome pathwayaureobasidin Aalpha-galactosidase - آلفا گالاکتوزیدازALP - آلکالن فسفاتازCardiac contractility - انقباض قلبیProtein–protein interaction - تعامل پروتئین-پروتئینUbiquitin-proteasome system - سیستم Ubiquitin-proteasomeFibrillin 1 - فیبریلین 1Filamin C - فیلامین سیLeft ventricular hypertrophy - هیپرتروفی بطن چپcardiac myosin binding protein C - پروتئین C اتصال مایوسین قلبیHypertrophic cardiomyopathy - کاردیومیوپاتی هایپرتروفیک UPS - یو پی اس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Myosin binding protein H (MyBPH) is a protein of unknown function, which shares sequence and structural similarities with myosin binding protein C (cMyBPC), a protein frequently implicated in hypertrophic cardiomyopathy (HCM). Given the similarity between cMyBPC and MyBPH, we proposed that MyBPH, like cMyBPC, could be involved in HCM pathogenesis and we therefore sought to determine its function. We identified MyBPH-interacting proteins by using yeast two-hybrid (Y2H) analysis. The role of MyBPH and cMyBPC in cardiac cell contractility was analysed by measuring the planar cell surface area of differentiated H9c2 rat cardiomyocytes in response to β-adrenergic stress after siRNA knockdown of MyBPH and cMyBPC. Individual knockdown of either protein had no effect on cardiac contractility, while concurrent knockdowns reduced cardiac contractility. These proteins therefore functionally compensate for one another and are critical for cardiac contractility. We further show that both proteins co-localise with the autophagosomal membrane protein LC3, suggesting that both proteins are involved in autophagosomal membrane maturation processes. The results of this study ascribe novel functions to MyBPH, which may contribute to our understanding of its role in the sarcomere. This study provides evidence for a potential role of MyBPH in HCM, which warrants further investigation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 331, Issue 2, 15 February 2015, Pages 338-351
Journal: Experimental Cell Research - Volume 331, Issue 2, 15 February 2015, Pages 338-351
نویسندگان
Jomien Mouton, Ben Loos, Johanna C Moolman-Smook, Craig J Kinnear,