کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904563 | 1086609 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Disassembly of the JIP1/JNK molecular scaffold by caspase-3-mediated cleavage of JIP1 during apoptosis
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کلمات کلیدی
NF-κBTNF Receptor Associated Death Domainreceptor interacting protein kinaseIB1MAPKK kinasezVAD.fmkTNFJIPMAPKKKMAPKKc-jun amino-terminal kinaseTRADDTRAFPARPFADDPBSJnkDTTGTPase Activating Protein - GTPase فعال کردن پروتئینMAPK - MAPKMAPK kinase - MAPK کینازEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetra-acetic acid - اتیلن دیامین تترا استیک اسیدFas-associated death domain - حوزه مرگ مرتبط FasApoptosis - خزان یاختهایdithiothreitol - دیتیوتریتولGAP - شکافPOSH - صبح بخیرtumour necrosis factor - عامل نکروز تومورTNF receptor associated factor - عامل گیرنده TNFnuclear factor κB - فاکتور هسته ای κBPhosphate buffered saline - فسفات بافر شورTRAIL - قطارTNF-related apoptosis inducing ligand - لیگاند القاء آپوپتوز مرتبط با TNFRIP - پاره کردنmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenCaspase-3 - کاسپاز ۳ Hela - کامل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Disassembly of the JIP1/JNK molecular scaffold by caspase-3-mediated cleavage of JIP1 during apoptosis Disassembly of the JIP1/JNK molecular scaffold by caspase-3-mediated cleavage of JIP1 during apoptosis](/preview/png/10904563.png)
چکیده انگلیسی
We report here the cleavage of the c-Jun N-terminal Kinase (JNK) pathway scaffold protein, JNK Interacting Protein-1 (JIP1), by caspases during both Tumour Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) and staurosporine-induced apoptosis in HeLa cells. During the initiation of apoptosis, maximal JNK activation is observed when JIP1 is intact, whereas cleavage of JIP1 correlates with JNK inactivation and progression of apoptosis. JIP1 is cleaved by caspase-3 at two sites, leading to disassembly of the JIP1/JNK complex. Inhibition of JIP1 cleavage by the caspase-3 inhibitor DEVD.fmk inhibits this disassembly, and is accompanied by sustained JNK activation. These data suggest that TRAIL and staurosporine induce JNK activation in a caspase-3-independent manner and that caspase-3-mediated JIP1 cleavage plays a role in JNK inactivation via scaffold disassembly during the execution phase of apoptosis. Caspase-mediated cleavage of JIP scaffold proteins may therefore represent an important mechanism for modulation of JNK signalling during apoptotic cell death.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 317, Issue 7, 15 April 2011, Pages 1028-1039
Journal: Experimental Cell Research - Volume 317, Issue 7, 15 April 2011, Pages 1028-1039
نویسندگان
Mahesh Vaishnav, Marion MacFarlane, Martin Dickens,