کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904769 | 1086693 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The ciliary rootlet interacts with kinesin light chains and may provide a scaffold for kinesin-1 vesicular cargos
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کلمات کلیدی
FRAPAPPJIPKHCPDIODFKLCHeptad repeat - Heptad تکرار کنیدpresenilin 1 - Presenilin 1tetratricopeptide repeat - tetratricopeptide تکرار کنیدTPR - روش پاسخ فیزیکیKinesin light chain - زنجیره سبک Kinesinkinesin heavy chain - زنجیره سنگین kinesinCiliated cells - سلولهای سرطانیPhotoreceptor - فتوتراپیfluorescence recovery after photobleaching - فلوئورسانس پس از فوتوبلاسیکOuter dense fibers - فیبرهای چگالی بیرونیamyloid precursor protein - پروتئین پیش ماده آمیلوئی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The ciliary rootlet is a large striated fibrous network originating from basal bodies in ciliated cells. To explore its postulated role in intracellular transport, we investigated the interaction between kinesin light chains (KLCs) and rootletin, the structural component of ciliary rootlets. We show here that KLCs directly interact with rootletin and are located along ciliary rootlets. Their interactions are mediated by the heptad repeats of KLCs. Further studies found that these interactions tethered kinesin heavy chains along ciliary rootlets. However, the ciliary rootlet-bound kinesin-1 did not recruit microtubules or move along ciliary rootlets. Additionally, amyloid precursor protein (APP; a kinesin-1 vesicular cargo receptor) and presenilin 1 (a presumed cargo of APP/kinesin-1) were found to be enriched along the rootletin fibers, suggesting that the interaction between ciliary rootlets and kinesin-1 recruits APP and presenilin 1 along ciliary rootlets. These findings indicate that ciliary rootlets may provide a scaffold for kinesin-1 vesicular cargos and, thus, play a role in the intracellular transport in ciliated cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 309, Issue 2, 1 October 2005, Pages 379-389
Journal: Experimental Cell Research - Volume 309, Issue 2, 1 October 2005, Pages 379-389
نویسندگان
Jun Yang, Tiansen Li,