کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10962663 | 1102646 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Strategic evaluation of vaccine candidate antigens for the prevention of Visceral Leishmaniasis
ترجمه فارسی عنوان
ارزیابی استراتژیک آنتی ژنهای کاندید واکسن برای جلوگیری از لیشمانیوز احشایی
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کلمات کلیدی
SMTGLA-SEH2BTLRMDHSterol methyltransferase - استرتول متیل ترانسفرازsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدProtozoa - تکیاختگانToll-like receptor - تیالآرCutaneous leishmaniasis - سالک، لیشمانیوز جلدی، لیشمانیوز پوستیT cell - سلول تیLeishmania - لیشمانیا Visceral leishmaniasis - لیشمانیاز احشایی، کالاآزارmalate dehydrogenase - ملات دهیدروژنازHistone H2B - هیستون H2BVaccine - واکسنProtein - پروتئین
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Infection with Leishmania parasites results in a range of clinical manifestations and outcomes, the most severe of which is visceral leishmaniasis (VL). Vaccination will likely provide the most effective long-term control strategy, as the large number of vectors and potential infectious reservoirs renders sustained interruption of Leishmania parasite transmission extremely difficult. Selection of the best vaccine is complicated because, although several vaccine antigen candidates have been proposed, they have emerged following production in different platforms. To consolidate the information that has been generated into a single vaccine platform, we expressed seven candidates as recombinant proteins in E. coli. After verifying that each recombinant protein could be recognized by VL patients, we evaluated their protective efficacy against experimental L. donovani infection of mice. Administration in formulation with the Th1-potentiating adjuvant GLA-SE indicated that each antigen could elicit antigen-specific Th1 responses that were protective. Considering the ability to reduce parasite burden along with additional factors such as sequence identity across Leishmania species, we then generated a chimeric fusion protein comprising a combination of the 8E, p21 and SMT proteins. This E. coli -expressed fusion protein was also demonstrated to protect against L. donovani infection. These data indicate a novel recombinant vaccine antigen with the potential for use in VL control programs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 34, Issue 25, 27 May 2016, Pages 2779-2786
Journal: Vaccine - Volume 34, Issue 25, 27 May 2016, Pages 2779-2786
نویسندگان
Malcolm S. Duthie, Michelle Favila, Kimberley A. Hofmeyer, Yeung L. Tutterrow, Steven J. Reed, John D. Laurance, Alessandro Picone, Jeffrey Guderian, H. Remy Bailor, Aarthy C. Vallur, Hong Liang, Raodoh Mohamath, Julie Vergara, Randall F. Howard,