کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10965884 | 1102762 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly immunostimulatory RNA derived from a Sendai virus defective viral genome
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کلمات کلیدی
RSVRLRsMDA5RIG-IDPSAdjuvant - ادجوانت، یاورImmunization - ایمن سازیinterferon - اینترفرونIFN - اینترفرون هاDendritic cells - سلول های دندریتیکSendai virus - ویروس SendaiMelanoma Differentiation-Associated protein 5 - پروتئین متمایز Melanoma-Associated 5Poly I:C - پلی I: CPolyinosinic:polycytidylic acid - پلی سونیک: پلیسییدیدیل اسیدSeV - چطوریretinoic acid-inducible gene 1 - ژن القاء شده اسید رتینوئیک 1RIG-I-like receptors - گیرنده هایی مانند RIG-I
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Defective viral genomes (DVGs) are generated during virus replication. DVGs bearing complementary ends are strong inducers of dendritic cell (DC) maturation and of the expression of antiviral and pro-inflammatory cytokines by triggering signaling of the RIG-I family of intracellular pattern recognition receptors. Our data show that DCs stimulated with virus containing DVGs have an enhanced ability to activate human T cells and can induce adaptive immunity in mice. In addition, we describe the generation of a short Sendai virus (SeV)-derived DVG RNA (DVG-324) that maintains strong immunostimulatory activity in vitro and in vivo. DVG-324 induced high levels of Ifnb expression when transfected into cells and triggered fast expression of pro-inflammatory cytokines and mobilization of dendritic cells when injected into the footpad of mice. Importantly, DVG-324 enhanced the production of antibodies to a prototypic vaccine after a single intramuscular immunization in mice. Notably, the pro-inflammatory cytokine profile induced by DVG-324 was different from that induced by poly I:C, the only viral RNA analog currently used as an immunostimulant in vivo, suggesting a distinct mechanism of action. SeV-derived oligonucleotides represent novel alternatives to be harnessed as potent adjuvants for vaccination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 48, 19 November 2013, Pages 5713-5721
Journal: Vaccine - Volume 31, Issue 48, 19 November 2013, Pages 5713-5721
نویسندگان
Xiomara Mercado-López, Christopher R. Cotter, Won-keun Kim, Yan Sun, Luis Muñoz, Karla Tapia, Carolina B. López,