کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10966518 | 1102779 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of immune response and protective effect of four vaccines against the tick-borne encephalitis virus
ترجمه فارسی عنوان
ارزیابی پاسخ ایمنی و اثر محافظتی چهار واکسن علیه ویروس آنسفالیت تلقی می شود
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کلمات کلیدی
TNFLethal dosesTick-borne flavivirusTCIDTBEvAFMLD50Protective effect - اثر محافظتیTem - این استEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاNeutralization test - تست خنثی سازیreverse transcription - رونویسی معکوسCytokines - سیتوکین هاtumor necrosis factor - فاکتور نکروز تومورhemagglutination inhibition - مهار هموگلوبینTransmission electron microscopy - میکروسکوپ الکترونی عبوریatomic force microscopy - میکروسکوپ نیروی اتمیInactivated vaccine - واکسن غیر فعال شدهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Among three main subtypes of the tick-borne encephalitis virus (TBEV), the Siberian subtype is currently dominant in a majority of the endemic regions of Russia. However, inactivated vaccines are based on TBEV strains of the heterologous Far Eastern or the European subtypes isolated 40-77 years ago. To analyze the efficacy of the available vaccines against currently prevailing TBEV isolates of the Siberian subtype, mice were immunized subcutaneously three times (one group per each vaccine). The expression of seven cytokine genes was determined using RT-PCR. Sera were studied using homologous and heterologous ELISA, hemagglutination inhibition (HI) and neutralization tests with TBEV strains of the Far Eastern, Siberian and European subtypes. Cross-protective efficacy of the vaccines was evaluated with the TBEV strain 2689 of Siberian subtype isolated from an ixodid tick from the Novosibirsk, South-Western Siberia, Russia in 2010. The cytokine gene expression profile indicates a predominantly Th2 response due to exogenous antigen presentation. Titers for homologous combinations of vaccine strain and strain in ELISA, HI and neutralization tests exceeded those for heterologous antigen-antibody pairs. Despite antibody detection by means of ELISA, HI and neutralization tests, the mouse protection afforded by the vaccines differed significantly. Complete protection of mice challenged with 100 LD50 virus of the Siberian subtype was induced by the vaccine “Encevir” (“Microgen”, Tomsk, Russia). The minimal immunization doze (MID50) of “Encevir” protecting 50% of the mice was less than 0.0016Â ml. Partial protective effect of vaccines produced in Moscow, Russia and Austria revealed MID50 within recommended intervals (0.001-0.017Â ml). However, the MID50 for the vaccine “Encepur” (Novartis, Germany) 0.04Â ml exceeded acceptable limits with total loss of mice immunized with vaccine diluted 32, 100 and 320 fold. These results suggest regular evaluation of TBEV vaccines in regions where heterologous virus subtypes prevail.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 25, 23 May 2014, Pages 3101-3106
Journal: Vaccine - Volume 32, Issue 25, 23 May 2014, Pages 3101-3106
نویسندگان
O.V. Morozova, V.N. Bakhvalova, O.F. Potapova, A.E. Grishechkin, E.I. Isaeva, K.V. Aldarov, D.V. Klinov, M.F. Vorovich,