کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10967478 | 1102832 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utility of the Sindbis replicon system as an Env-targeted HIV vaccine
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کلمات کلیدی
NspsHBSSENVICSPKRSIVHIV-1 envelope proteinRepliconSFVVEEVsin - بدونintracellular staining - رنگ آمیزی داخل سلولیSindbis - سندیبیزHank's balanced salt solution - محلول نمک متعادل هانکinfectious units - واحدهای عفونیVaccine - واکسنSemliki Forest virus - ویروس Semliki جنگلSindbis virus - ویروس SindbisVenezuelan equine encephalitis virus - ویروس آنسفالیت اسب ونزوئلاHIV - ویروس نقص ایمنی انسانی Simian immunodeficiency virus - ویروس کمبود ایمنی سیمانیEnvelope - پاکت نامهNon-structural proteins - پروتئین های غیر ساختاری
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sindbis replicon-based vaccine vectors are designed to combine the immunostimulatory properties of replicating viruses with the superior safety profile of non-replicating systems. In this study we performed a detailed assessment of Sindbis (SIN) replicon vectors expressing HIV-1 envelope protein (Env) for the induction of cell-mediated and humoral immune responses in a small animal model. SIN-derived virus-like particles (VLP) elicited Env-specific antibody responses that were detectable after boosting with recombinant Env protein. This priming effect could be mediated by replicon activity alone but may be enhanced by Env attached to the surface of VLP, offering a potential advantage for this mode of replicon delivery for Env based vaccination strategies. In contrast, the Env-specific CTL responses that were elicited by SIN-VLP were entirely dependent on replicon activity. SIN-VLP priming induced more durable humoral responses than immunization with protein only. This is important from a vaccine perspective, given the intrinsic tendency of Env to induce short-lived antibody responses in the context of vaccination or infection. These results indicate that further efforts to enhance the magnitude and durability of the HIV-1 Env-specific immune responses generated by Sindbis vectors, either alone or as part of prime-boost regimens, are justified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 31, Issue 18, 26 April 2013, Pages 2260-2266
Journal: Vaccine - Volume 31, Issue 18, 26 April 2013, Pages 2260-2266
نویسندگان
Rob J. Center, Annett Miller, Adam K. Wheatley, Shahan M. Campbell, Carly Siebentritt, Damian F.J. Purcell,