Enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: Linking systems biology with vaccine development

► We analyzed in vivo gene expression data from both the bovine host and the zoonotic bacterial pathogens. ► We developed computational systems biology models of the host-pathogen interactions. ► We applied Dynamic Bayesian Network machine learning to identify pathogenicity biosignatures. ► The systems biology analysis identified significant biosignature mechanistic genes as deletion targets for enhanced rational design and attenuation of live vaccine candidates.