کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1163695 | 1490979 | 2015 | 8 صفحه PDF | دانلود رایگان |
• An aptamer based QCM biosensor is developed for the determination of Salmonella.
• The aptamers on magnetic beads and QCM crystal chips has high specificity to Salmonella cells.
• The detection limit of the proposed method was observed about 10 CFU mL−1.
• This method with conjunction with magnetic beads system offers a rapid, and sensitive detection of Salmonella in complex medium.
• The binding of Salmonella cells to aptamer-sensor chip was well described Langmuir model.
A quartz crystal microbalance sensor (QCM) was developed for sensitive and specific detection of Salmonella enterica serovar typhimurium cells in food samples by integrating a magnetic bead purification system. Although many sensor formats based on bioaffinity agents have been developed for sensitive and specific detection of bacterial cells, the development of robust sensor applications for food samples remained a challenging issue. A viable strategy would be to integrate QCM to a pre-purification system. Here, we report a novel and sensitive high throughput strategy which combines an aptamer-based magnetic separation system for rapid enrichment of target pathogens and a QCM analysis for specific and real-time monitoring. As a proof-of-concept study, the integration of Salmonella binding aptamer immobilized magnetic beads to the aptamer-based QCM system was reported in order to develop a method for selective detection of Salmonella. Since our magnetic separation system can efficiently capture cells in a relatively short processing time (less than 10 min), feeding captured bacteria to a QCM flow cell system showed specific detection of Salmonella cells at 100 CFU mL−1 from model food sample (i.e., milk). Subsequent treatment of the QCM crystal surface with NaOH solution regenerated the aptamer-sensor allowing each crystal to be used several times.
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Journal: Analytica Chimica Acta - Volume 853, 1 January 2015, Pages 533–540