کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1166720 | 960509 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Pre-analytical method for NMR-based grape metabolic fingerprinting and chemometrics Pre-analytical method for NMR-based grape metabolic fingerprinting and chemometrics](/preview/png/1166720.png)
Although metabolomics aims at profiling all the metabolites in organisms, data quality is quite dependent on the pre-analytical methods employed. In order to evaluate current methods, different pre-analytical methods were compared and used for the metabolic profiling of grapevine as a model plant. Five grape cultivars from Portugal in combination with chemometrics were analyzed in this study. A common extraction method with deuterated water and methanol was found effective in the case of amino acids, organic acids, and sugars. For secondary metabolites like phenolics, solid phase extraction with C-18 cartridges showed good results. Principal component analysis, in combination with NMR spectroscopy, was applied and showed clear distinction among the cultivars. Primary metabolites such as choline, sucrose, and leucine were found discriminating for ‘Alvarinho’, while elevated levels of alanine, valine, and acetate were found in ‘Arinto’ (white varieties). Among the red cultivars, higher signals for citrate and GABA in ‘Touriga Nacional’, succinate and fumarate in ‘Aragonês’, and malate, ascorbate, fructose and glucose in ‘Trincadeira’, were observed. Based on the phenolic profile, ‘Arinto’ was found with higher levels of phenolics as compared to ‘Alvarinho’. ‘Trincadeira’ showed lowest phenolics content while higher levels of flavonoids and phenylpropanoids were found in ‘Aragonês’ and ‘Touriga Nacional’, respectively. It is shown that the metabolite composition of the extract is highly affected by the extraction procedure and this consideration has to be taken in account for metabolomics studies.
Pre-analytical method for NMR-based grape metabolic fingerprinting and chemometrics.Figure optionsDownload as PowerPoint slideHighlights
► Different pre-analytical methods were used for metabolic profiling of grapevine.
► We define own range of metabolites detected in each method.
► Extraction with deuterated solvents shows higher efficiency of primary metabolites.
► Solid phase extraction with C18 improves extraction efficiency of minor phenolics.
Journal: Analytica Chimica Acta - Volume 703, Issue 2, 10 October 2011, Pages 179–186