کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207501 1493715 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples
چکیده انگلیسی

A new in-capillary derivatization method with naphtalene-2,3-dicarboxyaldehyde (NDA)/CN− has been developed for capillary electrophoresis with laser-induced fluorescence detection of brain microdialysate amino acids. Samples are sandwiched between two plugs of reagent mixture at the capillary inlet and subsequently separated. Highest derivatization yields are obtained by using a reagent to sample plug length ratio equal to 4, performing a first electrophoretic mixing followed by a zero potential amplification step before applying the separation voltage and using a NaCN to NDA concentration ratio equal to 1. This new single-step methodology allows the analysis of amino acid neurotransmitters in rat brain microdialysis samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1205, Issues 1–2, 26 September 2008, Pages 144–149
نویسندگان
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