کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1422220 986441 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins
چکیده انگلیسی

ObjectivesThe aim of this study was to evaluate the cytotoxic effect of the monomers isobutyl methacrylate (IBMA) and 1,6-hexanediol dimethacrylate (1,6-HDMA), the plasticizer di-n-butyl phthalate (DBP), and the degradation by-products methacrylic acid (MA) and benzoic acid (BA) on L929 cells. Based on previous investigations on the release of these compounds from hard chairside reline resins, a range of concentrations (μmol/L) were selected for the cytotoxicity tests (IBMA, 5.49–1406.57; 1,6-HDMA, 1.22–39.32; DBP, 1.12–143.8; MA, 9.07–581; BA, 3.19–409).MethodsCytotoxic effects were assessed using MTT and 3H-thymidine assays after the cells had been exposed to the test compounds at the given concentrations for 24 h. Cytotoxicity was rated based on cell viability relative to controls (cells exposed to medium without test substances).ResultsDNA synthesis activity was inhibited by all compounds. Mitochondrial dehydrogenase activity decreased in cells treated with monomers, plasticizer and MA by-product, whereas no cytotoxic effect was observed on contact with BA at the majority of concentrations tested. The ranges of suppression for 3H-thymidine assay were: IBMA, 25–95%; 1,6-HDMA, 95–98%; DBP, 40–98%; MA, 97–99%; BA, 54–71%. For MTT assay, the ranges of suppression were: IBMA, 0–96%; 1,6-HDMA, 26–89%; DBP, 17–80%; MA, 52–66%; BA, 0–27%. The 3H-thymidine assay was more sensitive than the MTT assay.SignificanceThis study evaluated the cytotoxicity of a wide range of concentrations of monomers (IBMA and 1,6-HDMA), plasticizer (DBP) and degradation by-products (MA and BA), including those expected to be released from hard chairside reline resins. The differences observed in the cytotoxicity of these compounds, along with other properties, may assist the dental practitioners in the selection of reline materials with improved service life performance and low risk of adverse reactions in patients who wear relined dentures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dental Materials - Volume 26, Issue 10, October 2010, Pages 1017–1023
نویسندگان
, , , , , ,