کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1666332 | 1518070 | 2013 | 7 صفحه PDF | دانلود رایگان |
• Toremifene (TOR) in dipalmitoyl-phosphatidyl-glycerol (DPPG) monolayers
• Electrostatic interactions between DPPG and TOR form a 1:1 complex.
• TOR increases the fluidity of DPPG monolayers.
• Incorporation of TOR in the fluid phase of DPPG followed by fluorescence imaging
Langmuir monolayers of dipalmitoyl-phosphatidyl-glycerol (DPPG) containing toremifene (TOR), an antiestrogen drug derivative of tamoxifen, were prepared in order to study the interaction of the drug with the cell membrane. TOR is not surface active but it remains at the interface in DPPG rich monolayers anchored by electrostatic interaction with the anionic DPPG up to the equimolar composition. The fluidity of mixed monolayers increases up to the TOR mole fraction XTOR = 0.3, remaining practically invariant for higher compositions. Brewster angle microscopy shows that the TOR disturbs the DPPG organization and the liquid condensed (LC) domains of DPPG become undetectable for XTOR ≥ 0.4. Laser scanning confocal fluorescence microscopy images of the LB films doped with rhodamine B-piperazine amide dye confirm the progressive reduction in size of LC domains, from which TOR and rhodamine are excluded. The incorporation of TOR in DPPG monolayers up to the equimolar composition supports the formation of a TOR:DPPG complex (1:1) due to electrostatic interactions between the negatively charged polar groups of DPPG and protonated TOR.
Journal: Thin Solid Films - Volume 534, 1 May 2013, Pages 584–590