کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910842 | 1046789 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional significance of the GAG trinucleotide-repeat polymorphism in the gene for the catalytic subunit of γ-glutamylcysteine ligase
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کلمات کلیدی
catalytic subunit of γ-glutamylcysteine ligaseuntranslated region.Short tandem repeat polymorphismSDSGCLMGCLCmRNAGAGGCLNADPHMPAGSHRBCRed blood cells - سلولهای قرمز خونDNA - DNA یا اسید دزوکسی ریبونوکلئیکAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدdeoxyribonucleic acid - اسید deoxyribonucleicmeta-phosphoric acid - اسید متا فسفریکmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدγ-glutamylcysteine ligase - لیگاز γ-گلوتامیل سیستئینUTR یا untranslated regions - منطقه ترجمه نشدهHemoglobin - هموگلوبینhigh-pressure liquid chromatography - کروماتوگرافی مایع با فشار بالاHPLC - کروماتوگرافی مایعی کاراGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional significance of the GAG trinucleotide-repeat polymorphism in the gene for the catalytic subunit of γ-glutamylcysteine ligase Functional significance of the GAG trinucleotide-repeat polymorphism in the gene for the catalytic subunit of γ-glutamylcysteine ligase](/preview/png/1910842.png)
چکیده انگلیسی
γ-Glutamylcysteine ligase (GCL) is the rate-limiting enzyme in glutathione (GSH) synthesis. A GAG-repeat polymorphism in the 5ⲠUTR of the gene coding for the catalytic subunit of GCL (GCLC) has been associated with altered GSH levels in vitro. Thus, we hypothesized that this polymorphism is associated with altered GCL activity and blood GSH levels in vivo. A total of 256 healthy United States black and white adults were genotyped for the GAG polymorphism and blood GSH levels were measured. In a subset of 107 individuals, blood GCL activity was determined. Five alleles with 4, 7, 8, 9, and 10 GAG repeats were observed. The most prevalent genotype was 7/9 (40%) followed by 7/7 (32%) and 9/9 (11%). GSH levels were 15% lower in 9/9 individuals than 7/9 individuals (P = 0.05). GCL activity was 21% lower in 9/9 individuals than 7/7 individuals (P = 0.04). A decreasing trend of GCL activity was observed in the order of 7/7 > 7/9 > 9/9 (P = 0.04). These findings show that 9/9 individuals have lower blood GSH levels, which is likely due to a decrease in GCL activity. Such individuals might be more susceptible to oxidative stress-related diseases than individuals with other genotypes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 45, Issue 5, 1 September 2008, Pages 645-650
Journal: Free Radical Biology and Medicine - Volume 45, Issue 5, 1 September 2008, Pages 645-650
نویسندگان
Sailendra N. Nichenametla, Irina Ellison, Ana Calcagnotto, Philip Lazarus, Josh E. Muscat, John P. Jr.,