کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928101 | 1050312 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV
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کلمات کلیدی
FBSDSBNHEJDAPIXRCC4DNA-PKcsLIG4NLS4,6-diamidino-2-phenylindole dihydrochloride - 4،6-دیامیدینو-2-فنیلینول دی هیدروکلرایدDNA ligase IV - DNA لیگاز IVDNA double-strand break repair - تعمیر DNA شکست دو رشتهfetal bovine serum - سرم جنین گاوnuclear localization signal - سیگنال محلی سازی هسته ایdouble-strand break - شکست دو ردیفnon-homologous end-joining - عدم پیوستن به همولوگNuclear localization - محلی سازی هسته ایUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهDNA-dependent protein kinase catalytic subunit - وابسته به DNA وابسته به پروتئین کیناز کاتالیزوری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV](/preview/png/1928101.png)
چکیده انگلیسی
XRCC4 and DNA Ligase IV (LIG4) cooperate to join two DNA ends at the final step of DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ). However, it is not fully understood how these proteins are localized to the nucleus. Here we created XRCC4K271R mutant, as Lys271 lies within the putative nuclear localization signal (NLS), and XRCC4K210R mutant, as Lys210 was reported to undergo SUMOylation, implicated in the nuclear localization of XRCC4. Wild-type and mutated XRCC4 with EGFP tag were introduced into HeLa cell, in which endogenous XRCC4 had been knocked down using siRNA directed to 3â²-untranslated region, and tested for the nuclear localization function by fluorescence microscopy. XRCC4K271R was defective in the nuclear localization of itself and LIG4, whereas XRCC4K210R was competent for the nuclear localization with LIG4. To examine DSB repair function, wild-type and mutated XRCC4 were introduced into XRCC4-deficient M10. M10-XRCC4K271R, but not M10-XRCC4K210R, showed significantly reduced surviving fraction after 2 Gy γ-ray irradiation as compared to M10-XRCC4WT. The number of γ-H2AX foci remaining 2 h after 2 Gy γ-ray irradiation was significantly greater in M10-XRCC4K271R than in M10-XRCC4WT, whereas it was only marginally increased in M10-XRCC4K210R as compared to M10-XRCC4WT. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 461, Issue 4, 12 June 2015, Pages 687-694
Journal: Biochemical and Biophysical Research Communications - Volume 461, Issue 4, 12 June 2015, Pages 687-694
نویسندگان
Mikoto Fukuchi, Rujira Wanotayan, Sicheng Liu, Shoji Imamichi, Mukesh Kumar Sharma, Yoshihisa Matsumoto,