کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1934494 | 1050643 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glucolipotoxicity in INS-1E cells is counteracted by carnitine palmitoyltransferase 1 over-expression Glucolipotoxicity in INS-1E cells is counteracted by carnitine palmitoyltransferase 1 over-expression](/preview/png/1934494.png)
Effects of non-esterified fatty acids (FAs) are accentuated when applied together with elevated glucose through preferential use of glucose as fuel, which leads to decreased oxidation of FAs. We examined how over-expression of the mitochondrial FA transporter carnitine palmitoyltransferase 1 (CPT1) affects glucose-stimulated insulin secretion (GSIS), apoptosis and ER stress in INS-1E cells cultured in the presence of elevated levels of glucose and palmitate. INS-1E cells were infected with Tet-ON regulated adenovirus containing CPT1 and cultured for 48 h in the presence of 0.5 mM palmitate and 20 mM glucose. Over-expressing CPT1 lowered basal insulin secretion in a dose-dependent manner thereby improving GSIS from INS-1E cells. Also, apoptosis was alleviated and ER-stress markers p-eIF2α and CHOP were decreased in cells over-expressing CPT1. We conclude that regulated over-expression of CPT1 is beneficial for glucolipotoxic beta-cells.
Journal: Biochemical and Biophysical Research Communications - Volume 375, Issue 4, 31 October 2008, Pages 517–521