کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935594 1050670 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Free fatty acids sensitize hepatocytes to bile acid-induced apoptosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Free fatty acids sensitize hepatocytes to bile acid-induced apoptosis
چکیده انگلیسی

Delivery of free fatty acids to the liver in nonalcoholic fatty liver disease (NAFLD) may render hepatocytes more vulnerable to glycochenodeoxycholic acid (GCDCA)-induced apoptosis. Fat overloading was induced in HepG2-Ntcp cells and primary rat hepatocytes by incubation with palmitic or oleic acid. Apoptosis was quantified by measuring caspase 3/7 activity and transcription of interleukin (IL) 8 and IL-22 by quantitative real-time PCR. Oleic acid (500 μM) alone did not induce apoptosis, while palmitic acid (500 μM) increased apoptosis 5-fold. GCDCA did not induce significant apoptosis at low micromolar concentrations (5–30 μM) in non-steatotic cells. However, at the same concentrations, GCDCA increased apoptosis 3-fold in oleic acid-pretreated HepG2-Ntcp cells and 3.5-fold in primary rat hepatocytes. Pretreatment with oleic acid increased GCDCA-induced gene transcription of the proinflammatory cytokines IL-8 and IL-22 5-fold and 19-fold, respectively. Thus, low levels of cholestasis normally not considered harmful could advance liver injury in patients with NAFLD.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 371, Issue 3, 4 July 2008, Pages 441–445
نویسندگان
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