کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939704 1050765 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gliotoxin induces caspase-dependent neurite degeneration and calpain-mediated general cytotoxicity in differentiated human neuroblastoma SH-SY5Y cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Gliotoxin induces caspase-dependent neurite degeneration and calpain-mediated general cytotoxicity in differentiated human neuroblastoma SH-SY5Y cells
چکیده انگلیسی

In this study, a significant increase by 50% in intracellular free calcium concentration ([Ca2+]i) was observed in differentiated human neuroblastoma (SH-SY5Y) cells after exposure to 0.25 μM of the fungal metabolite gliotoxin for 72 h. Further, the involvement of caspases and calpains was demonstrated to underlie the gliotoxin-induced cytotoxic and neurite degenerative effects. The caspase inhibitor Z-VAD-fmk almost completely reduced the neurite degeneration from 40% degeneration of neurites to 5% as compared to control. Inhibition of calpains with calpeptin significantly attenuated gliotoxin-induced cytotoxicity, determined as reduction in total cellular protein content, from 43% to 14% as compared to control cells. Western blot analyses of αII-spectrin breakdown fragments confirmed activity of the proteases, and that αII-spectrin was cleaved by caspases in gliotoxin-exposed cells. These results show that calpains and caspases have a role in the toxicity of gliotoxin in differentiated SH-SY5Y cells and that the process may be Ca2+-mediated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 345, Issue 3, 7 July 2006, Pages 1068–1074
نویسندگان
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