کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1943385 | 1052662 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II
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کلمات کلیدی
NeoxanthinViolaxanthinTMFNEOLUTChln-Dodecyl-β-D-maltoside - n-Dodecyl-β-D-مالتوزیدVio - ارهReconstitution - بازسازیThermostability - ترموستاتاcircular dichroism - رنگ تابی دورانیChlorophyll - سبزینه یا کلروفیلPhotosystem - سیستم عکسPhotostability - عکسبرداریLutein - لوتئین، ویتامین چشمCAR - ماشینCarotenoid - کاروتنوئیدhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II](/preview/png/1943385.png)
چکیده انگلیسی
Three isoforms of the major light-harvesting chlorophyll (Chl) a/b complexs of photosystem II (LHCIIb) in the pea, namely, Lhcb1, Lhcb2, and Lhcb3, were obtained by overexpression of apoprotein in Escherichia coli and by successfully refolding these isoforms with thylakoid pigments in vitro. The sequences of the protein, pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different isoforms were analysed. Comparison of their spectroscopic properties and structural stabilities revealed that Lhcb3 differed strongly from Lhcb1 and Lhcb2 in both respects. It showed the lowest Qy transition energy, with its reddest absorption about 2 nm red-shifted, and the highest photostability under strong illuminations. Among the three isoforms, Lhcb 2 showed lowest thermal stability regarding energy transfer from Chl b to Chl a in the complexes, which implies that the main function of Lhcb 2 under high temperature stress is not the energy transfer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1777, Issue 6, June 2008, Pages 479-487
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1777, Issue 6, June 2008, Pages 479-487
نویسندگان
Yajie Zhang, Cheng Liu, Shuang Liu, Ye Shen, Tingyun Kuang, Chunhong Yang,