کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1964547 | 1058557 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential activation of the PI 3-kinase effectors AKT/PKB and p70 S6 kinase by compound 48/80 is mediated by PKCα
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کلمات کلیدی
C48/80tPAmTORp70 S6 kinaseNCSPFAPDK-1phorbol 12-myristate 13-acetatePKCPKBPI 3-kinase - PI 3-کینازBAPTA - بیایپیتیایbisindolylmaleimide I - بیستینولیل مولارین ICompound 48/80 - ترکیب 48/80newborn calf serum - سرم گاو تازه متولد شدهphosphoinositide 3-kinase - فسفینوزیتید 3-کینازBIM - مدلسازی اطلاعات ساختمانmammalian target of rapamycin - هدف پستانداران رپامایسینPhosphoinositide-dependent kinase-1 - وابسته به فسفوینوزیته kinase-1paraformaldehyde - پارافرمالدهیدprotein kinase B - پروتئین کیناز BProtein kinase C - پروتئین کیناز سیPten - ژن PTEN
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The secretagogue compound 48/80 (c48/80) is a well known activator of calcium mediated processes and PKCs, and is a potent inducer of mast cell degranulation. As the latter process is a phosphoinositide 3-kinase (PI 3-kinase) mediated event, we wished to address whether or not c48/80 was an activator of PI 3-kinases. The data presented here reveal that c48/80 is an effective activator of PI 3-kinases as judged by the increased phosphorylation of PKB and p70S6K in fibroblasts in a PI 3-kinase dependent fashion. Compound 48/80 effectively translocates PKB to the plasma membrane and induces phosphorylation at serine 473 (S473), detected by fluorescence imaging of fixed cells. At higher concentrations the secretagogue is inhibitory towards PKB phosphorylation on S473. Conversely, p70S6K phosphorylation on T389 is unaffected at high doses. We provide evidence that the differential effect on the two PI 3-kinase effectors is due to activation of PKCα by c48/80, itself a PI 3-kinase dependent process. We conclude that compound 48/80 is an effective activator of PI 3-kinase dependent pathways, leading to the activation of effectors including PKB/Akt, p70S6K and PKCα. The latter is only activated by higher doses of c48/80 resulting in an inhibition of the c48/80 induced PKB phosphorylation, thus explaining the observed biphasic activation profile for PKB in response to this secretagogue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 19, Issue 2, February 2007, Pages 321-329
Journal: Cellular Signalling - Volume 19, Issue 2, February 2007, Pages 321-329
نویسندگان
Richard D. Byrne, Erika Rosivatz, Maddy Parsons, Banafshé Larijani, Peter J. Parker, Tony Ng, Rudiger Woscholski,