کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015574 | 1541930 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional profiling by DDRT-PCR analysis reveals gene expression during seed development in Carya cathayensis Sarg.
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کلمات کلیدی
TDFsDifferential mRNA displayC4HGene differential expressionDDRT-PCRNADH dehydrogenase subunit 1nad1Succinyl-CoA ligaseDAPBAPTFsMUFAPUFAsFatty acid desaturase - اسید لاکتیک اسیدFatty acid - اسید چربPolyunsaturated fatty acids - اسید چرب اشباع نشدهmonounsaturated fatty acid - اسید چرب غیر اشباعFAD - بدtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولtubulin - توبولینdays after pollination - روز بعد از گردهافشانیFatty acid synthesis - سنتز اسید چربcinnamate 4-hydroxylase - سینامات 4-هیدروکسیلازTranscription factors - عوامل رونویسیSAD - غمگینTranscript-derived fragments - قطعات مشتق شده از متنGene ontology - هستیشناسی ژنیtub - وان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hickory (Carya cathayensis Sarg.) seed has one of the highest oil content and is rich in polyunsaturated fatty acids (PUFAs), which kernel is helpful to human health, particularly to human brain function. A better elucidation of lipid accumulation mechanism would help to improve hickory production and seed quality. DDRT-PCR analysis was used to examine gene expression in hickory at thirteen time points during seed development process. A total of 67 unique genes involved in seed development were obtained, and those expression patterns were further confirmed by semi-quantitative RT-PCR and real time RT-PCR analysis. Of them, the genes with known functions were involved in signal transduction, amino acid metabolism, nuclear metabolism, fatty acid metabolism, protein metabolism, carbon metabolism, secondary metabolism, oxidation of fatty acids and stress response, suggesting that hickory underwent a complex metabolism process in seed development. Furthermore, 6 genes related to fatty acid synthesis were explored, and their functions in seed development process were further discussed. The data obtained here would provide the first clues for guiding further functional studies of fatty acid synthesis in hickory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 91, June 2015, Pages 28-35
Journal: Plant Physiology and Biochemistry - Volume 91, June 2015, Pages 28-35
نویسندگان
You-Jun Huang, Qin Zhou, Jian-Qin Huang, Yan-Ru Zeng, Zheng-Jia Wang, Qi-Xiang Zhang, Yi-Hang Zhu, Chen Shen, Bing-Song Zheng,