کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016023 | 1541935 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced accumulation of carotenoids in sweetpotato plants overexpressing IbOr-Ins gene in purple-fleshed sweetpotato cultivar
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کلمات کلیدی
ABACRTIFlavanone-3-hydroxylaseCCDSZEPLCY-βPSYGGPPCaMVPDSDFRZDSF3HASANCED9-cis-epoxycarotenoid dioxygenase - 9-cis-epoxycarotinoid dioxygenaseβ-carotene hydroxylase - β-کاروتن هیدروکسیلازAscorbic acid - آسکوربیک اسیدAnthocyanin - آنتوسیانینabscisic acid - اسید آبسزیکzeaxanthin epoxidase - اکسیداز zeaxanthindihydroflavonol 4-reductase - دی هیدروفلوانول 4-ردوکتازcarotenoid cleavage dioxygenases - دیاکسژنازهای جداسازی کاروتنوئیدZeaxanthin - زاکسانتیینANS - سالanthocyanidin synthase - سنتاز آنتوسیانیدینphytoene synthase - سنتاز فیتوئنSweetpotato - سیب زمینی شیرینGAP - شکافPhytoene desaturase - فیتون دساتورازlycopene β-cyclase - لیکوپن β-سیکلاسdry weight - وزن خشکcauliflower mosaic virus - ویروس موزائیک گل کلمCarotenoid - کاروتنوئیدglyceraldehyde-3-phosphate - گلیسرالیدید-3-فسفاتgeranylgeranyl diphosphate - گوارانیلگرانیل دی فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sweetpotato [Ipomoea batatas (L.) Lam] is an important root crop that produces low molecular weight antioxidants such as carotenoids and anthocyanin. The sweetpotato orange (IbOr) protein is involved in the accumulation of carotenoids. To increase the levels of carotenoids in the storage roots of sweetpotato, we generated transgenic sweetpotato plants overexpressing IbOr-Ins under the control of the cauliflower mosaic virus (CaMV) 35S promoter in an anthocyanin-rich purple-fleshed cultivar (referred to as IbOr plants). IbOr plants exhibited increased carotenoid levels (up to 7-fold) in their storage roots compared to wild type (WT) plants, as revealed by HPLC analysis. The carotenoid contents of IbOr plants were positively correlated with IbOr transcript levels. The levels of zeaxanthin were â¼12 times elevated in IbOr plants, whereas β-carotene increased â¼1.75 times higher than those of WT. Quantitative RT-PCR analysis revealed that most carotenoid biosynthetic pathway genes were up-regulated in the IbOr plants, including PDS, ZDS, LCY-β, CHY-β, ZEP and Pftf, whereas LCY-É was down-regulated. Interestingly, CCD1, CCD4 and NCED, which are related to the degradation of carotenoids, were also up-regulated in the IbOr plants. Anthocyanin contents and transcription levels of associated biosynthetic genes seemed to be altered in the IbOr plants. The yields of storage roots and aerial parts of IbOr plants and WT plants were not significantly different under field cultivation. Taken together, these results indicate that overexpression of IbOr-Ins can increase the carotenoid contents of sweetpotato storage roots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 86, January 2015, Pages 82-90
Journal: Plant Physiology and Biochemistry - Volume 86, January 2015, Pages 82-90
نویسندگان
Sung-Chul Park, Sun Ha Kim, Seyeon Park, Hyeong-Un Lee, Joon Seol Lee, Woo Sung Park, Mi-Jeong Ahn, Yun-Hee Kim, Jae Cheol Jeong, Haeng-Soon Lee, Sang-Soo Kwak,