کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2506838 | 1557532 | 2006 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of the anti-neoplastic agent ET-18-OCH3 and some analogs on the biophysical properties of model membranes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MLVsinterdigitated gel phaseLβ′LβILα31P-NMRLUVsDEPEDSCMOPSPKCdMPCLPC31P NMR31P-nuclear magnetic resonance1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-هیدروکسی-اس-گلیسرو-3-فسفوکلین1,2-dimyristoyl-sn-glycero-3-phosphocholine - 1،2-dimyristoyl-sn-glycero-3-phosphosocholineChemical shift anisotropy - Anisotropy شبیه سازی شیمیاییET-18-OCH3 - ET-18-OCH 3PAPc - PAPCmultilamellar vesicles - vesicles multilamellarEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدHII - اینlarge unilamellar vesicles - بزرگ کیسه های بدون انعطاف پذیرTransition temperature - دمای انتقالModel membranes - غشاهای مدلLamellar liquid crystalline phase - فاز بلوری مایع لمینالInverted hexagonal phase - فاز شش ضلعی متناوبlamellar gel phase - فاز ژل لایه ایPlatelet-activating factor - فاکتور فعال کننده پلاکتphosphatidyl ethanolamine - فسفاتیدیل اتانولامینPAF - نیروی هوایی پاکستانProtein kinase C - پروتئین کیناز سیDifferential scanning calorimetry - کالریمتری روبشی افتراقی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
علوم دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effect of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (ET-18-OCH3, edelfosine), and six other analog asymmetric phosholipids on the physical properties of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) model membranes was studied using differential scanning calorimetry (DSC), 31P-nuclear magnetic resonance (31P NMR) and X-ray diffraction. DSC data revealed that, at concentrations of 40 mol% and higher, a new type of mixtures with higher Tc and narrower transitions appeared with all the asymmetric lipids studied. At very high concentrations of these lipids (50-80 mol%), destabilization was observed in the systems probably because of the formation of micelles or small vesicles. In all cases, the asymmetric lipids at concentrations of 40 mol% induced the formation of interdigitated structures in the lamellar gel phase, as deduced from X-ray diffraction. The asymmetric phospholipids were also added to 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine (DEPE) model membranes and DSC data revealed that the lipids primarily affected transition from the lamellar gel (Lβ) to the lamellar liquid crystalline (Lα) phase in two aspects: the transition temperature was reduced, and the transition itself became broader and smaller. The lamellar liquid crystalline (Lα) to inverted hexagonal phase (HII) transition was also affected, as detected by DSC and 31P NMR data. Increasing concentrations of the asymmetric lipids reduced the formation of inverted hexagonal phases, which were completely inhibited in the case of ET-18-OCH3. Since these compounds have been shown to have important biological actions through the plasma membrane, these results may help to understand the mechanism of action of these compounds. In addition these asymmetric lipids were tested for their capacity to induce cell apoptosis, and only ET-18-OCH3 was found to have a clear effect, thus suggesting that the apoptotic effect is not exerted through changes in the biophysical properties of model membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 318, Issues 1â2, 2 August 2006, Pages 28-40
Journal: International Journal of Pharmaceutics - Volume 318, Issues 1â2, 2 August 2006, Pages 28-40
نویسندگان
Alejandro Torrecillas, J. Daniel Aroca-Aguilar, Francisco J. Aranda, Consuelo Gajate, Faustino Mollinedo, Senena Corbalán-GarcÃa, Ana de Godos, Juan C. Gómez-Fernández,